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Histidine-rich glyc...
Histidine-rich glycoprotein blocks collagen-binding integrins and adhesion of endothelial cells through low-affinity interaction with alpha 2 integrin
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- Roche, Francis (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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Sipilä, Kalle (author)
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Honjo, Satoshi (author)
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- Staffan, Johansson (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Staffan Johansson
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- Tugues, Sonia (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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Heino, Jyrki (author)
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- Claesson-Welsh, Lena (author)
- Uppsala universitet,Vaskulärbiologi
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(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- English.
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In: Matrix Biology. - : Elsevier BV. - 0945-053X .- 1569-1802. ; 48, s. 89-99
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The plasma protein histidine-rich glycoprotein (HRG) affects the morphology and function of both endothelial cells (ECs) and monocytes/macrophages in cancer. Here, we examined the mechanism of action of HRG's effect on ECs. HRG suppressed adhesion, spreading and migration of ECs specifically on collagen I (COL I) whereas ECs seeded on other extracellular matrix proteins were insensitive to HRG. HRG did not bind specifically to COL I or to the α-integrin binding site on collagen, GFOGER. Furthermore, HRG's inhibition of EC adhesion was not dependent upon heparan sulfate (HS) moieties as heparitinase-treated ECs remained sensitive to HRG. C2C12 cells expressing α2 integrin, the major collagen-binding α-integrin subunit in ECs, showed increased binding of HRG compared with wild type C2C12 cells lacking the α2 subunit. Recombinant α2 I-domain protein bound HRG and to a higher extent when in active conformation. However, the α2 I-domain bound weakly to HRG compared with COL I and the purified α2β1 ectodomain complex failed to retain HRG. We conclude that HRG binds to α2 integrin through low-affinity interactions in a HS-independent manner, thereby blocking EC-adhesion to COL I.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- HRG; Adhesion; Integrin; Endothelial cells; Collagen
Publication and Content Type
- ref (subject category)
- art (subject category)
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