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Insulitis and chara...
Insulitis and characterisation of infiltrating T cells in surgical pancreatic tail resections from patients at onset of type 1 diabetes
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Krogvold, Lars (author)
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- Wiberg, Anna (author)
- Uppsala universitet,Klinisk immunologi,Korsgren
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Edwin, Bjørn (author)
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Buanes, Trond (author)
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Jahnsen, Frode Lars (author)
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Hanssen, Kristian F (author)
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- Larsson, Erik (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Klinisk patologi
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- Korsgren, Olle (author)
- Uppsala universitet,Klinisk immunologi
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- Skog, Oskar (author)
- Uppsala universitet,Klinisk immunologi
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Dahl-Jørgensen, Knut (author)
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(creator_code:org_t)
- 2015-11-24
- 2016
- English.
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In: Diabetologia. - : Springer Science and Business Media LLC. - 0012-186X .- 1432-0428. ; 59:3, s. 492-501
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https://link.springe...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Aims/hypothesisIt is thought that T cells play a major role in the immune-mediated destruction of beta cells in type 1 diabetes, causing inflammation of the islets of Langerhans (insulitis). The significance of insulitis at the onset of type 1 diabetes is debated, and the role of the T cells poorly understood.MethodsIn the Diabetes Virus Detection (DiViD) study, pancreatic tissue from six living patients with recent-onset type 1 diabetes was collected. The insulitis was characterised quantitatively by counting CD3+ T cells, and qualitatively by transcriptome analysis targeting 84 T and B lymphocyte genes of laser-captured microdissected islets. The findings were compared with gene expression in T cells collected from kidney biopsies from allografts with ongoing cellular rejection. Cytokine and chemokine release from isolated islets was characterised and compared with that from islets from non-diabetic organ donors.ResultsAll six patients fulfilled the criteria for insulitis (5–58% of the insulin-containing islets in the six patients had ≥ 15 T cells/islet). Of all the islets, 36% contained insulin, with several resembling completely normal islets. The majority (61–83%) of T cells were found as peri-insulitis rather than within the islet parenchyma. The expression pattern of T cell genes was found to be markedly different in islets compared with the rejected kidneys. The islet-infiltrating T cells showed only background levels of cytokine/chemokine release in vitro.Conclusions/interpretationInsulitis and a significant reserve reservoir for insulin production were present in all six cases of recent-onset type 1 diabetes. Furthermore, the expression patterns and levels of cytokines argue for a different role of the T cells in type 1 diabetes when compared with allograft rejection.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Keyword
- Gene expression; Inflammation; Insulin; Insulitis; Pancreas; T cells; Type 1 diabetes
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Krogvold, Lars
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Wiberg, Anna
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Edwin, Bjørn
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Buanes, Trond
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Jahnsen, Frode L ...
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Hanssen, Kristia ...
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show more...
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Larsson, Erik
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Korsgren, Olle
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Skog, Oskar
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Dahl-Jørgensen, ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Endocrinology an ...
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Diabetologia
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Uppsala University