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Characterization of...
Characterization of preproenkephalin expressing neurons in the nervous system using a transgenic line
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- Aresh, Bejan, 1984- (author)
- Uppsala universitet,Genetisk utvecklingsbiologi,Sensory Circuits
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- Moelijker, Nynke (author)
- Uppsala universitet,Genetisk utvecklingsbiologi,Sensory Circuits
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- Blümel, Edda (author)
- Uppsala universitet,Genetisk utvecklingsbiologi,Sensory Circuits
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- Lagerström, Malin (author)
- Uppsala universitet,Genetisk utvecklingsbiologi,Sensory Circuits
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(creator_code:org_t)
- English.
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
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- Here we have constructed a Cre line to target preproenkephalin expressing cells (Penk-Cre) using a BAC cloning strategy. By crossing our Penk-Cre line with the tdTomato reporter, tdTomato;Penk-Cre expressing cells could be visualized. Penk-Cre was expressed throughout the dorsal spinal cord, where approximately 50% of the neurons were residing within lamina III. Furthermore, single-cell analysis of spinal Penk-Cre expressing cells showed that 41% was positive for Penk mRNA and that a majority (94%) of the population expressed Vglut2 mRNA. Immunohistochemical analysis showed that only 7% and 13% expressed the inhibitory markers Viaat-egfp and Pax2, respectively, hence identifying spinal Penk-Cre expressing neurons as mainly excitatory. The expression of Penk-Cre in the brain was extensive, including dense expression in the striatum, nucleus accumbens and several amygdaloid nuclei. Furthermore, Penk-Cre expression was also shown in insular cortex, cingulated cortex, primary and secondary somatosensory cortices and the trigeminal nuclei. The Penk-Cre line represents a useful genetic tool for future analysis of PENK expressing neurons in the nervous system.
Keyword
- characterization
- Cre
- preproenkephalin
- Penk
- Medicinsk vetenskap
- Medical Science
Publication and Content Type
- vet (subject category)
- ovr (subject category)
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