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Blockade of vascula...
Blockade of vascular endothelial growth factor receptor-3 signaling inhibits fibroblast growth factor-2-induced lymphangiogenesis in mouse cornea.
- Article/chapterEnglish2002
Publisher, publication year, extent ...
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2002-06-17
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Proceedings of the National Academy of Sciences,2002
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-287168
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-287168URI
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https://doi.org/10.1073/pnas.062040199DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1947203URI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Vascular endothelial growth factor receptor-3 (VEGFR-3) is a major mediator of lymphangiogenesis. Recently, VEGFR-3 ligands, VEGF-C, and VEGF-D were reported to promote tumor lymphangiogenesis and lymphatic metastasis, and these processes were inhibited by blocking of the VEGFR-3-signaling pathway. Here, we have adapted the mouse corneal angiogenesis assay to study potential lymphangiogenic factors and inhibitors. Immunohistochemical analysis with lymphatic endothelial markers showed that VEGF-C induces lymphatic as well as blood vessel growth in the cornea. By contrast, VEGF induced angiogenesis but not lymphangiogenesis. Fibroblast growth factor-2 (FGF-2) stimulated both lymphangiogenesis and angiogenesis. FGF-2 up-regulated VEGF-C expression in vascular endothelial and perivascular cells. Furthermore, administration of blocking anti-VEGFR-3 antibodies inhibited the FGF-2-induced lymphangiogenesis. These findings show that VEGFR-3 can mediate lymphangiogenesis induced by other growth factors. Because increased expression of FGF-2 and VEGF-C has been associated with lymphatic metastasis, our results provide a potential strategy for the inhibition of lymphatic metastasis in cancer therapy.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Cao, RenhaiKarolinska Institutet
(author)
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Brakenhielm, Ebba
(author)
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Mäkinen, Taija
(author)
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Cao, YihaiKarolinska Institutet
(author)
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Alitalo, Kari
(author)
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Karolinska Institutet
(creator_code:org_t)
Related titles
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In:Proceedings of the National Academy of Sciences of the United States of America: Proceedings of the National Academy of Sciences99:130027-84241091-6490
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