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Simultaneous imagin...
Simultaneous imaging of multiple neurotransmitters and neuroactive substances in the brain by desorption electrospray ionization mass spectrometry
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- Shariatgorji, Mohammadreza (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Strittmatter, Nicole (author)
- AstraZeneca, Drug Safety & Metab, Cambridge CB4 0WG, England.
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- Nilsson, Anna (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Kallbäck, Patrik (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Alvarsson, Alexandra (author)
- Karolinska Inst, Ctr Mol Med, Dept Neurol & Clin Neurosci, S-17176 Stockholm, Sweden.;Karolinska Univ Hosp, S-17176 Stockholm, Sweden.
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- Zhang, Xiaoqun (author)
- Karolinska Institutet
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- Vallianatou, Theodosia (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Svenningsson, Per (author)
- Karolinska Institutet
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- Goodwin, Richard J. A. (author)
- AstraZeneca, Drug Safety & Metab, Cambridge CB4 0WG, England.
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- Andrén, Per E. (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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(creator_code:org_t)
- Elsevier BV, 2016
- 2016
- English.
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In: NeuroImage. - : Elsevier BV. - 1053-8119 .- 1095-9572. ; 136, s. 129-138
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- With neurological processes involving multiple neurotransmitters and neuromodulators, it is important to have the ability to directly map and quantify multiple signaling molecules simultaneously in a single analysis. By utilizing a molecular-specific approach, namely desorption electrospray ionization mass spectrometry imaging (DESI-MSI), we demonstrated that the technique can be used to image multiple neurotransmitters and their metabolites (dopamine, dihydroxyphenylacetic acid, 3-methoxytyramine, serotonin, glutamate, glutamine, aspartate,gamma-aminobutyric acid, adenosine) as well as neuroactive drugs (amphetamine, sibutramine, fluvoxamine) and drug metabolites in situ directly in brain tissue sections. The use of both positive and negative ionization modes increased the number of identified molecular targets. Chemical derivatization by charge-tagging the primary amines of molecules significantly increased the sensitivity, enabling the detection of low abundant neurotransmitters and other neuroactive substances previously undetectable by MSI. The sensitivity of the imaging approach of neurochemicals has a great potential in many diverse applications in fields such as neuroscience, pharmacology, drug discovery, neurochemistry, and medicine.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Radiologi och bildbehandling (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Radiology, Nuclear Medicine and Medical Imaging (hsv//eng)
Keyword
- Neurotransmitter
- Dopamine
- Imaging
- Mass spectrometry
- Parkinson's disease
- Serotonin
Publication and Content Type
- ref (subject category)
- art (subject category)
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NeuroImage
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Shariatgorji, Mo ...
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Strittmatter, Ni ...
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Nilsson, Anna
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Kallbäck, Patrik
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Alvarsson, Alexa ...
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Zhang, Xiaoqun
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Vallianatou, The ...
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Svenningsson, Pe ...
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Goodwin, Richard ...
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Andrén, Per E.
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Neurology
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MEDICAL AND HEAL ...
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NeuroImage
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Uppsala University
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Karolinska Institutet