Simultaneous imaging of multiple neurotransmitters and neuroactive substances in the brain by desorption electrospray ionization mass spectrometry

Shariatgorji, MohammadrezaUppsala universitet,Institutionen för farmaceutisk biovetenskap (author)

With neurological processes involving multiple neurotransmitters and neuromodulators, it is important to have the ability to directly map and quantify multiple signaling molecules simultaneously in a single analysis. By utilizing a molecular-specific approach, namely desorption electrospray ionization mass spectrometry imaging (DESI-MSI), we demonstrated that the technique can be used to image multiple neurotransmitters and their metabolites (dopamine, dihydroxyphenylacetic acid, 3-methoxytyramine, serotonin, glutamate, glutamine, aspartate,gamma-aminobutyric acid, adenosine) as well as neuroactive drugs (amphetamine, sibutramine, fluvoxamine) and drug metabolites in situ directly in brain tissue sections. The use of both positive and negative ionization modes increased the number of identified molecular targets. Chemical derivatization by charge-tagging the primary amines of molecules significantly increased the sensitivity, enabling the detection of low abundant neurotransmitters and other neuroactive substances previously undetectable by MSI. The sensitivity of the imaging approach of neurochemicals has a great potential in many diverse applications in fields such as neuroscience, pharmacology, drug discovery, neurochemistry, and medicine.

Strittmatter, NicoleAstraZeneca, Drug Safety & Metab, Cambridge CB4 0WG, England. (author)
Nilsson, AnnaUppsala universitet,Institutionen för farmaceutisk biovetenskap(Swepub:uu)annil134 (author)
Kallbäck, PatrikUppsala universitet,Institutionen för farmaceutisk biovetenskap(Swepub:uu)joaka138 (author)
Alvarsson, AlexandraKarolinska Inst, Ctr Mol Med, Dept Neurol & Clin Neurosci, S-17176 Stockholm, Sweden.;Karolinska Univ Hosp, S-17176 Stockholm, Sweden. (author)
Zhang, XiaoqunKarolinska Institutet (author)
Vallianatou, TheodosiaUppsala universitet,Institutionen för farmaceutisk biovetenskap(Swepub:uu)theva108 (author)
Svenningsson, PerKarolinska Institutet (author)
Goodwin, Richard J. A.AstraZeneca, Drug Safety & Metab, Cambridge CB4 0WG, England. (author)
Andrén, Per E.Uppsala universitet,Institutionen för farmaceutisk biovetenskap(Swepub:uu)perandre (author)
Uppsala universitetInstitutionen för farmaceutisk biovetenskap (creator_code:org_t)

By the author/editor: Shariatgorji, Mo ...; Strittmatter, Ni ...; Nilsson, Anna; Kallbäck, Patrik; Alvarsson, Alexa ...; Zhang, Xiaoqun; show more...; Vallianatou, The ...; Svenningsson, Pe ...; Goodwin, Richard ...; Andrén, Per E.; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Neurology

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Radiology Nuclea ...

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