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A data set from fla...
A data set from flash X-ray imaging of carboxysomes
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- Hantke, Max F. (author)
- Uppsala universitet,Molekylär biofysik
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- Hasse, Dirk (author)
- Uppsala universitet,Molekylär biofysik
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- Ekeberg, Tomas (author)
- Uppsala universitet,Molekylär biofysik
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- John, Katja (author)
- Uppsala universitet,Molekylär biofysik
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- Svenda, Martin (author)
- Uppsala universitet,Molekylär biofysik
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Loh, Duane (author)
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Martin, Andrew V. (author)
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- Timneanu, Nicusor (author)
- Uppsala universitet,Molekylär biofysik,Molekyl- och kondenserade materiens fysik
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- Larsson, Daniel S.D. (author)
- Uppsala universitet,Molekylär biofysik
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- van der Schot, Gijs (author)
- Uppsala universitet,Molekylär biofysik
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- Carlsson, Gunilla H. (author)
- Uppsala universitet,Molekylär biofysik
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- Ingelman, Margareta (author)
- Uppsala universitet,Molekylär biofysik
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- Andreasson, Jakob (author)
- Uppsala universitet,Molekylär biofysik
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- Westphal, Daniel (author)
- Uppsala universitet,Molekylär biofysik
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- Iwan, Bianca (author)
- Uppsala universitet,Molekylär biofysik
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- Uetrecht, Charlotte (author)
- Uppsala universitet,Molekylär biofysik
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- Bielecki, Johan (author)
- Uppsala universitet,Molekylär biofysik
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Liang, Mengning (author)
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Stellato, Francesco (author)
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DePonte, Daniel P. (author)
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Bari, Sadia (author)
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Hartmann, Robert (author)
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Kimmel, Nils (author)
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Kirian, Richard A. (author)
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- Seibert, M. Marvin (author)
- Uppsala universitet,Molekylär biofysik
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- Mühlig, Kerstin (author)
- Uppsala universitet,Molekylär biofysik
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Schorb, Sebastian (author)
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Ferguson, Ken (author)
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Bostedt, Christoph (author)
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Carron, Sebastian (author)
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Bozek, John D. (author)
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Rolles, Daniel (author)
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Rudenko, Artem (author)
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Foucar, Lutz (author)
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Epp, Sascha W. (author)
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Chapman, Henry N. (author)
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Barty, Anton (author)
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- Andersson, Inger (author)
- Uppsala universitet,Molekylär biofysik
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- Hajdu, Janos (author)
- Uppsala universitet,Molekylär biofysik
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- Maia, Filipe R.N.C. (author)
- Uppsala universitet,Molekylär biofysik
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(creator_code:org_t)
- 2016-08-01
- 2016
- English.
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In: Scientific Data. - : Springer Science and Business Media LLC. - 2052-4463. ; 3
- Related links:
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https://uu.diva-port... (primary) (Raw object)
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
Close
- Ultra-intense femtosecond X-ray pulses from X-ray lasers permit structural studies on single particles and biomolecules without crystals. We present a large data set on inherently heterogeneous, polyhedral carboxysome particles. Carboxysomes are cell organelles that vary in size and facilitate up to 40% of Earth’s carbon fixation by cyanobacteria and certain proteobacteria. Variation in size hinders crystallization. Carboxysomes appear icosahedral in the electron microscope. A protein shell encapsulates a large number of Rubisco molecules in paracrystalline arrays inside the organelle. We used carboxysomes with a mean diameter of 115±26 nm from Halothiobacillus neapolitanus. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min. Every diffraction pattern is a unique structure measurement and high-throughput imaging allows sampling the space of structural variability. The different structures can be separated and phased directly from the diffraction data and open a way for accurate, high-throughput studies on structures and structural heterogeneity in biology and elsewhere.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Hantke, Max F.
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Hasse, Dirk
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Ekeberg, Tomas
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John, Katja
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Svenda, Martin
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Loh, Duane
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show more...
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Martin, Andrew V ...
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Timneanu, Nicuso ...
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Larsson, Daniel ...
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van der Schot, G ...
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Carlsson, Gunill ...
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Ingelman, Margar ...
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Andreasson, Jako ...
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Westphal, Daniel
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Iwan, Bianca
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Uetrecht, Charlo ...
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Bielecki, Johan
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Liang, Mengning
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Stellato, France ...
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DePonte, Daniel ...
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Bari, Sadia
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Hartmann, Robert
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Kimmel, Nils
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Kirian, Richard ...
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Seibert, M. Marv ...
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Mühlig, Kerstin
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Schorb, Sebastia ...
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Ferguson, Ken
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Bostedt, Christo ...
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Carron, Sebastia ...
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Bozek, John D.
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Rolles, Daniel
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Rudenko, Artem
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Foucar, Lutz
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Epp, Sascha W.
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Chapman, Henry N ...
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Barty, Anton
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Andersson, Inger
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Hajdu, Janos
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Maia, Filipe R.N ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
- Articles in the publication
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Scientific Data
- By the university
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Uppsala University