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Comparison of Flow Cytometry and Image-Based Screening for Cell Cycle Analysis
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- Matuszewski, Damian J. (author)
- Uppsala universitet,Bildanalys och människa-datorinteraktion,Science for Life Laboratory, SciLifeLab
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- Sintorn, Ida-Maria (author)
- Uppsala universitet,Bildanalys och människa-datorinteraktion,Science for Life Laboratory, SciLifeLab
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- Puigvert, Jordi Carreras (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Division of Translational Medicine and Chemical Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
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- (creator_code:org_t)
- 2016-07-01
- 2016
- English.
- In: Image Analysis And Recognition (ICIAR 2016). - Cham : Springer. ; , s. 623-630
- Conference paper (peer-reviewed)
Abstract
Subject headings
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- Quantitative cell state measurements can provide a wealth of information about mechanism of action of chemical compounds and gene functionality. Here we present a comparison of cell cycle disruption measurements from commonly used flow cytometry (generating onedimensional signal data) and bioimaging (producing two-dimensional image data). Our results show high correlation between the two approaches indicating that image-based screening can be used as an alternative to flow cytometry. Furthermore, we discuss the benefits of image informatics over conventional single-signal flow cytometry.
Subject headings
- NATURVETENSKAP -- Data- och informationsvetenskap -- Bioinformatik (hsv//swe)
- NATURAL SCIENCES -- Computer and Information Sciences -- Bioinformatics (hsv//eng)
Keyword
- Quantitative microscopy
- DNA content histogram
Publication and Content Type
- ref (subject category)
- kon (subject category)
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