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Gene-specific corre...
Gene-specific correlation of RNA and protein levels in human cells and tissues
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- Edfors, Fredrik (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,KTH Royal Inst Technol, Sci Life Lab, Stockholm, Sweden.,Uhlen
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- Danielsson, Frida (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,KTH Royal Inst Technol, Sci Life Lab, Stockholm, Sweden.
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- Hallström, Björn M. (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,KTH Royal Inst Technol, Sci Life Lab, Stockholm, Sweden.
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- Käll, Lukas (author)
- KTH,Genteknologi,Science for Life Laboratory, SciLifeLab,KTH Royal Inst Technol, Sci Life Lab, Stockholm, Sweden.,Proteomik och nanobioteknologi
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- Lundberg, Emma (author)
- KTH,Science for Life Laboratory, SciLifeLab,KTH Royal Inst Technol, Sci Life Lab, Stockholm, Sweden.,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab
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- Pontén, Fredrik (author)
- Uppsala universitet,Klinisk och experimentell patologi,Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden
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- Forsström, Björn (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,KTH Royal Inst Technol, Sci Life Lab, Stockholm, Sweden.
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- Uhlén, Mathias (author)
- KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab,Technical University of Denmark, Denmark,KTH Royal Inst Technol, Sci Life Lab, Stockholm, Sweden.;Tech Univ Denmark, Novo Nordisk Fdn Ctr Biosustainabil, Horsholm, Denmark.
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(creator_code:org_t)
- 2016-10-26
- 2016
- English.
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In: Molecular Systems Biology. - : EMBO. - 1744-4292 .- 1744-4292. ; 12:10
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Abstract
Subject headings
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- An important issue for molecular biology is to establish whether transcript levels of a given gene can be used as proxies for the corresponding protein levels. Here, we have developed a targeted proteomics approach for a set of human non-secreted proteins based on parallel reaction monitoring to measure, at steady-state conditions, absolute protein copy numbers across human tissues and cell lines and compared these levels with the corresponding mRNA levels using transcriptomics. The study shows that the transcript and protein levels do not correlate well unless a gene-specific RNA-to-protein (RTP) conversion factor independent of the tissue type is introduced, thus significantly enhancing the predictability of protein copy numbers from RNA levels. The results show that the RTP ratio varies significantly with a few hundred copies per mRNA molecule for some genes to several hundred thousands of protein copies per mRNA molecule for others. In conclusion, our data suggest that transcriptome analysis can be used as a tool to predict the protein copy numbers per cell, thus forming an attractive link between the field of genomics and proteomics.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- gene expression
- protein quantification
- targeted proteomics
- transcriptomics
- Bioteknologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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