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Allergen-loaded str...
Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice
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- Garbani, M. (author)
- Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich
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- Xia, Wei (author)
- Uppsala universitet,Tillämpad materialvetenskap,Materials in Medcine
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- Rhyner, C. (author)
- Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich
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- Prati, M. (author)
- Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich
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- Scheynius, Annika (author)
- Karolinska Institutet
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- Malissen, B. (author)
- Centre d’Immunologie de Marseille-Luminy (CIML), INSERM
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- Engqvist, Håkan, 1972- (author)
- Uppsala universitet,Tillämpad materialvetenskap
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- Maurer, M. (author)
- Department of Dermatology and Allergy, Charité -Universitätsmedizin Berlin,
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- Crameri, Reto (author)
- Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich,
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- Terhorst-Molawi, Dorothea (author)
- UM2 Aix Marseille Univ, INSERM, U1104, CNRS,UMR7280,CIML, Marseille 9, France.; Charite, Dept Dermatol & Allergy, Allergie Ctr Charite, Berlin, Germany.; BIH, Berlin, Germany
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(creator_code:org_t)
- 2016-12-22
- 2017
- English.
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In: Allergy. European Journal of Allergy and Clinical Immunology. - : Wiley. - 0105-4538 .- 1398-9995. ; 72:4, s. 570-578
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- BackgroundImmunomodulatory interventions play a key role in the treatment of infections and cancer as well as allergic diseases. Adjuvants such as micro- and nanoparticles are often added to immunomodulatory therapies to enhance the triggered immune response. Here, we report the immunological assessment of novel and economically manufactured microparticle adjuvants, namely strontium-doped hydroxyapatite porous spheres (SHAS), which we suggest for the use as adjuvant and carrier in allergen-specific immunotherapy (ASIT).Methods and ResultsScanning electron microscopy revealed that the synthesis procedure developed for the production of SHAS results in a highly homogeneous population of spheres. SHAS bound and released proteins such as ovalbumin (OVA) or the major cat allergen Fel d 1. SHAS-OVA were taken up by human monocyte-derived dendritic cells (mdDCs) and murine DCs and did not have any necrotic or apoptotic effects even at high densities. In a murine model of ASIT for allergic asthmatic inflammation we found that OVA released from subcutaneously injected SHAS-OVA led to a sustained stimulation of both CD4+ and CD8+ T-cells. ASIT with SHAS-OVA as compared to soluble OVA resulted in similar humoral responses but in a higher efficacy as assessed by symptom scoring.ConclusionWe conclude that SHAS may constitute a suitable carrier and adjuvant for ASIT with great potential due to its unique protein-binding properties.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk material- och protesteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Materials (hsv//eng)
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- ref (subject category)
- art (subject category)
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Garbani, M.
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Xia, Wei
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Rhyner, C.
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Prati, M.
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Scheynius, Annik ...
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Malissen, B.
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show more...
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Engqvist, Håkan, ...
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Maurer, M.
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Crameri, Reto
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Terhorst-Molawi, ...
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Medical Engineer ...
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and Medical Material ...
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Allergy. Europea ...
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Uppsala University
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Karolinska Institutet