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Integrating mass sp...
Integrating mass spectrometry with MD simulations reveals the role of lipids in Na+/H+ antiporters
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Landreh, Michael (author)
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- Marklund, Erik G (author)
- Uppsala universitet,Biokemi,Erik Marklund
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- Uzdavinys, Povilas (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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Degiacomi, Matteo T. (author)
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- Coincon, Mathieu (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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Gault, Joseph (author)
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Gupta, Kallol (author)
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Liko, Idlir (author)
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Benesch, Justin L. P. (author)
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- Drew, David (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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Robinson, Carol V. (author)
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(creator_code:org_t)
- 2017-01-10
- 2017
- English.
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In: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 8
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Abstract
Subject headings
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- Na+/H+ antiporters are found in all kingdoms of life and exhibit catalysis rates that are among the fastest of all known secondary-active transporters. Here we combine ion mobility mass spectrometry and molecular dynamics simulations to study the conformational stability and lipid-binding properties of the Na+/H+ exchanger NapA from Thermus thermophilus and compare this to the prototypical antiporter NhaA from Escherichia coli and the human homologue NHA2. We find that NapA and NHA2, but not NhaA, form stable dimers and do not selectively retain membrane lipids. By comparing wild-type NapA with engineered variants, we show that the unfolding of the protein in the gas phase involves the disruption of inter-domain contacts. Lipids around the domain interface protect the native fold in the gas phase by mediating contacts between the mobile protein segments. We speculate that elevator-type antiporters such as NapA, and likely NHA2, use a subset of annular lipids as structural support to facilitate large-scale conformational changes within the membrane.
Subject headings
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
- NATURVETENSKAP -- Data- och informationsvetenskap -- Bioinformatik (hsv//swe)
- NATURAL SCIENCES -- Computer and Information Sciences -- Bioinformatics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Biologi med inriktning mot strukturbiologi
- Biology with specialization in Structural Biology
- Biochemistry
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Landreh, Michael
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Marklund, Erik G
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Uzdavinys, Povil ...
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Degiacomi, Matte ...
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Coincon, Mathieu
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Gault, Joseph
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show more...
-
Gupta, Kallol
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Liko, Idlir
-
Benesch, Justin ...
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Drew, David
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Robinson, Carol ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Structural Biolo ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Computer and Inf ...
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and Bioinformatics
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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Nature Communica ...
- By the university
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Uppsala University
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Stockholm University