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Local checkpoint in...
Local checkpoint inhibition of CTLA-4 as a monotherapy or in combination with anti-PD1 prevents the growth of murine bladder cancer
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- van Hooren, Luuk (author)
- Uppsala University,Uppsala universitet,Vaskulärbiologi
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- Sandin, Linda C. (author)
- Uppsala University,Uppsala universitet,Klinisk immunologi
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- Moskalev, Igor (author)
- Vancouver Prostate Centre, University of British Columbia, Vancouver, Canada
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- Ellmark, Peter (author)
- Lund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH,Alligator Biosciences AB
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- Dimberg, Anna (author)
- Uppsala University,Uppsala universitet,Vaskulärbiologi
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- Black, Peter (author)
- Alligator Bioscience AB, Lund, Sweden
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- Tötterman, Thomas (author)
- Uppsala University,Uppsala universitet,Klinisk immunologi
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- Mangsbo, Sara (author)
- Uppsala University,Uppsala universitet,Klinisk immunologi
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(creator_code:org_t)
- 2016-12-13
- 2017
- English.
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In: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 47:2, s. 385-393
- Related links:
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http://dx.doi.org/10...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Checkpoint blockade of CTLA-4 results in long-lasting survival benefits in metastatic cancer patients. However, patients treated with CTLA-4 blockade have suffered from immune-related adverse events, most likely due to the breadth of the induced T-cell activation. Here, we investigated the efficacy of a local low-dose anti-CTLA-4 administration for treatment of subcutaneous or orthotopic murine bladder 49 (MB49) bladder carcinoma in C57BL/6 mice. When MB49 tumors were grown s.c., peritumoral (p.t.) injection of anti-CTLA-4 treatment was equally effective as intravenous or s.c. (nontumor bearing flank) administration. Notably, p.t. injection was associated with lower circulating antibody levels and decreased IL-6 serum levels as compared to systemic treatment. Ultrasound-guided intratumoral anti-CTLA-4 antibody treatment of orthotopically growing MB49 tumors resulted in tumor regression, with more than tenfold reduction in systemic antibody levels as compared to i.v. or s.c. administration, in line with the compartmentally restrained nature of the bladder. Local anti-CTLA-4 therapy in combination with anti-PD-1 therapy resulted in complete responses, superior to each therapy alone. In addition, p.t. anti-CTLA-4 therapy was potentiated by depletion of regulatory T cells. Our results demonstrate that local anti-CTLA-4 antibody therapy is equally effective as systemic administration, but reduces systemic antibody levels and cytokine release, and enhances the response to anti-PD1 therapy.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- Bladder cancer
- CTLA-4
- Checkpoint inhibitors
- Immunotherapy
- Local low-dose
- MB49
- PD-1
- Bladder cancer
- Checkpoint inhibitors
- CTLA-4
- Immunotherapy
- Local low-dose
- MB49
- PD-1
Publication and Content Type
- ref (subject category)
- art (subject category)
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