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Loss of DNA-binding...
Loss of DNA-binding and new transcriptional trans-activation function in polyomavirus large T-antigen with mutation of zinc finger motif.
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Bergqvist, A (author)
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Nilsson, M (author)
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- Bondeson, K (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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Magnusson, G (author)
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(creator_code:org_t)
- 1990
- 1990
- English.
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In: Nucleic Acids Research. - 0305-1048 .- 1362-4962. ; 18:9, s. 2715-20
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
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- A putative zinc finger in polyomavirus large T-antigen was investigated. We were unable to demonstrate unequivocally a requirement for zinc in specific DNA-binding using the chelating agent 1, 10-phenanthroline. An involvement of the putative zinc finger in specific DNA-binding was nevertheless suggested by the properties of a mutant protein with a cys----ser replacement in the finger motif. Probably as a result of the defective DNA-binding, the mutant protein had lost its activity in initiation of viral DNA-replication and in negative regulation of viral early transcription. However, the trans-activation of the viral late promoter was normal. The analysis also revealed a previously unrecognized activity of large T-antigen. The mutant protein trans-activated the viral early promoter. In the wild-type protein this activity is probably concealed by the separate, negative regulatory function.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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