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  • Shubin, Nicholas J.Seattle Childrens Res Inst, Ctr Immun & Immunotherapies, 1900 9th Ave,Rm 721, Seattle, WA 98101 USA. (author)

Proteome analysis of mast cell releasates reveals a role for chymase in the regulation of coagulation factor XIIIA levels via proteolytic degradation

  • Article/chapterEnglish2017

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  • MOSBY-ELSEVIER,2017
  • printrdacarrier

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  • LIBRIS-ID:oai:DiVA.org:uu-319689
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-319689URI
  • https://doi.org/10.1016/j.jaci.2016.03.051DOI
  • https://res.slu.se/id/publ/88936URI

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  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

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  • Background: Mast cells are significantly involved in IgE-mediated allergic reactions; however, their roles in health and disease are incompletely understood. Objective: We aimed to define the proteome contained in mast cell releasates on activation to better understand the factors secreted by mast cells that are relevant to the contribution of mast cells in diseases. Methods: Bone marrow-derived cultured mast cells (BMCMCs) and peritoneal cell-derived mast cells were used as "surrogates'' for mucosal and connective tissue mast cells, respectively, and their releasate proteomes were analyzed by mass spectrometry. Results: Our studies showed that BMCMCs and peritoneal cell-derived mast cells produced substantially different releasates following IgE-mediated activation. Moreover, we observed that the transglutaminase coagulation factor XIIIA (FXIIIA) was one of the most abundant proteins contained in the BMCMC releasates. Mast cell-deficient mice exhibited increased FXIIIA plasma and activity levels as well as reduced bleeding times, indicating that mast cells are more efficient in their ability to downregulate FXIIIA than in contributing to its amounts and functions in homeostatic conditions. We found that human chymase and mouse mast cell protease-4 (the mouse homologue of human chymase) had the ability to reduce FXIIIA levels and function via proteolytic degradation. Moreover, we found that chymase deficiency led to increased FXIIIA amounts and activity, as well as reduced bleeding times in homeostatic conditions and during sepsis. Conclusions: Our study indicates that the mast cell protease content can shape its releasate proteome. Moreover, we found that chymase plays an important role in the regulation of FXIIIA via proteolytic degradation.

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  • Glukhova, Veronika A.Seattle Childrens Res Inst, Ctr Immun & Immunotherapies, 1900 9th Ave,Rm 721, Seattle, WA 98101 USA. (author)
  • Clauson, MorganSeattle Childrens Res Inst, Ctr Immun & Immunotherapies, 1900 9th Ave,Rm 721, Seattle, WA 98101 USA. (author)
  • Truong, PhuongSeattle Childrens Res Inst, Ctr Immun & Immunotherapies, 1900 9th Ave,Rm 721, Seattle, WA 98101 USA. (author)
  • Åbrink, MagnusSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health,Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, Uppsala, Sweden.(Swepub:slu)48145 (author)
  • Pejler, GunnarSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Swedish Univ Agr Sci, Dept Anat Physiol & Biochem, Uppsala, Sweden.,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB),Uppsala University(Swepub:slu)49530 (author)
  • White, Nathan J.Univ Washington, Dept Med, Div Emergency Med, Seattle, WA USA. (author)
  • Deutsch, Gail H.Seattle Childrens Res Inst, Dept Labs, Seattle, WA USA. (author)
  • Reeves, Stephen R.Seattle Childrens Res Inst, Ctr Immun & Immunotherapies, 1900 9th Ave,Rm 721, Seattle, WA 98101 USA.;Univ Washington, Dept Pediat, Seattle, WA 98195 USA. (author)
  • Vaisar, TomasUniv Washington, Dept Med, Div Metab Endocrinol & Nutr, Seattle, WA USA. (author)
  • James, Richard G.Seattle Childrens Res Inst, Ctr Immun & Immunotherapies, 1900 9th Ave,Rm 721, Seattle, WA 98101 USA. (author)
  • Piliponsky, Adrian M.Seattle Childrens Res Inst, Ctr Immun & Immunotherapies, 1900 9th Ave,Rm 721, Seattle, WA 98101 USA.;Univ Washington, Dept Pediat, Seattle, WA 98195 USA. (author)
  • Seattle Childrens Res Inst, Ctr Immun & Immunotherapies, 1900 9th Ave,Rm 721, Seattle, WA 98101 USA.Inst för biomedicin och veterinär folkhälsovetenskap (creator_code:org_t)
  • Sveriges lantbruksuniversitet

Related titles

  • In:Journal of Allergy and Clinical Immunology: MOSBY-ELSEVIER139:1, s. 323-3340091-67491097-6825

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