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Ubiquitously expres...
Ubiquitously expressed Human Beta Defensin 1 (hBD1) forms bacteria-entrapping nets in a redox dependent mode of action
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- Raschig, Judith (författare)
- Univ Hosp Tuebingen, Internal Med 1, Tubingen, Germany.
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- Mailaender-Sanchez, Daniela (författare)
- Univ Hosp Tuebingen, Internal Med 1, Tubingen, Germany.
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- Berscheid, Anne (författare)
- Univ Tubingen, Interfac Inst Microbiol & Infect Med, Dept Microbial Bioact Cpds, Tubingen, Germany.
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- Berger, Juergen (författare)
- Max Planck Inst Dev Biol, Electron Microscopy, Tubingen, Germany.
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- Strömstedt, Adam A. (författare)
- Uppsala universitet,Avdelningen för farmakognosi
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- Courth, Lioba F. (författare)
- Univ Hosp Tuebingen, Internal Med 1, Tubingen, Germany.
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- Malek, Nisar P. (författare)
- Univ Hosp Tuebingen, Internal Med 1, Tubingen, Germany.
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- Broetz-Oesterhelt, Heike (författare)
- Univ Tubingen, Interfac Inst Microbiol & Infect Med, Dept Microbial Bioact Cpds, Tubingen, Germany.
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- Wehkamp, Jan (författare)
- Univ Hosp Tuebingen, Internal Med 1, Tubingen, Germany.
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Univ Hosp Tuebingen, Internal Med 1, Tubingen, Germany Univ Tubingen, Interfac Inst Microbiol & Infect Med, Dept Microbial Bioact Cpds, Tubingen, Germany. (creator_code:org_t)
- 2017-03-21
- 2017
- Engelska.
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Ingår i: PLoS Pathogens. - : PUBLIC LIBRARY SCIENCE. - 1553-7366 .- 1553-7374. ; 13:3
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https://journals.plo...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Ever since the discovery of endogenous host defense antimicrobial peptides it has been discussed how these evolutionary conserved molecules avoid to induce resistance and to remain effective. Human beta-defensin 1 (hBD1) is an ubiquitously expressed endogenous antimicrobial peptide that exhibits qualitatively distinct activities between its oxidized and reduced forms. Here, we explore these antimicrobial mechanisms. Surprisingly, using electron microscopy we detected a so far unknown net-like structure surrounding bacteria, which were treated with the reduced but not the oxidized form of hBD1. A transmigration assay demonstrated that hBD1-derived nets capture bacteria and inhibit bacterial transmigration independent of bacterial killing. The presence of nets could completely prevent migration of hBD1 resistant pathogens and are stable in the presence of human duodenal secretion with a high amount of proteases. In contrast to HD6, cysteins are necessary for net formation. This redox-dependent function serves as an additional mechanism of action for hBD1 and differs from net formation by other defensins such as Paneth cell-derived human alpha-defensin 6 (HD6). While hBD1red and hBD1ox have distinct antimicrobial profiles and functions, only the reduced form provides additional host protection by entrapping bacteria in extracellular net structures preventing bacterial invasion. Better understanding of the modes of action of endogenous host peptides will help to find new antimicrobial strategies.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
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