Search: onr:"swepub:oai:DiVA.org:uu-323459" >
Combined lipidomic ...
-
Roomp, KirstenUniv Luxembourg, Luxembourg Ctr Syst Biomed, Esch Belval, Luxembourg.
(author)
Combined lipidomic and proteomic analysis of isolated human islets exposed to palmitate reveals time-dependent changes in insulin secretion and lipid metabolism
- Article/chapterEnglish2017
Publisher, publication year, extent ...
-
2017-04-27
-
Public Library of Science (PLoS),2017
-
electronicrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:uu-323459
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-323459URI
-
https://doi.org/10.1371/journal.pone.0176391DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Studies on the pathophysiology of type 2 diabetes mellitus (T2DM) have linked the accumulation of lipid metabolites to the development of beta-cell dysfunction and impaired insulin secretion. In most in vitro models of T2DM, rodent islets or beta-cell lines are used and typically focus is on specific cellular pathways or organs. Our aim was to, firstly, develop a combined lipidomics and proteomics approach for lipotoxicity in isolated human islets and, secondly, investigate if the approach could delineate novel and/or confirm reported mechanisms of lipotoxicity. To this end isolated human pancreatic islets, exposed to chronically elevated palmitate concentrations for 0, 2 and 7 days, were functionally characterized and their levels of multiple targeted lipid and untargeted protein species determined. Glucosestimulated insulin secretion from the islets increased on day 2 and decreased on day 7. At day 7 islet insulin content decreased and the proinsulin to insulin content ratio doubled. Amounts of cholesterol, stearic acid, C16 dihydroceramide and C24: 1 sphingomyelin, obtained from the lipidomic screen, increased time-dependently in the palmitate-exposed islets. The proteomic screen identified matching changes in proteins involved in lipid biosynthesis indicating up-regulated cholesterol and lipid biosynthesis in the islets. Furthermore, proteins associated with immature secretory granules were decreased when palmitate exposure time was increased despite their high affinity for cholesterol. Proteins associated with mature secretory granules remained unchanged. Pathway analysis based on the protein and lipid expression profiles implicated autocrine effects of insulin in lipotoxicity. Taken together the study demonstrates that combining different omics approaches has potential in mapping of multiple simultaneous cellular events. However, it also shows that challenges exist for effectively combining lipidomics and proteomics in primary cells. Our findings provide insight into how saturated fatty acids contribute to islet cell dysfunction by affecting the granule maturation process and confirmation in human islets of some previous findings from rodent islet and cell-line studies.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Kristinsson, HjaltiUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)hjakr151
(author)
-
Schvartz, DomitilleUniv Geneva, Human Prot Sci Dept, Ctr Med Univ, Geneva, Switzerland.
(author)
-
Ubhayasekera, KumariUppsala universitet,Analytisk kemi,Science for Life Laboratory, SciLifeLab(Swepub:uu)kumub884
(author)
-
Sargsyan, ErnestUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)ernsa357
(author)
-
Manukyan, LevonUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)levma108
(author)
-
Chowdhury, Azazul IslamUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)azach262
(author)
-
Manell, HannesUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)hanoh982
(author)
-
Satagopam, VenkataUniv Luxembourg, Luxembourg Ctr Syst Biomed, Esch Belval, Luxembourg.
(author)
-
Groebe, KarlfriedPivot Biomed Sci GmbH, Trier, Germany.
(author)
-
Schneider, ReinhardUniv Luxembourg, Luxembourg Ctr Syst Biomed, Esch Belval, Luxembourg.
(author)
-
Bergquist, JonasUppsala universitet,Analytisk kemi,Science for Life Laboratory, SciLifeLab(Swepub:uu)jbe25356
(author)
-
Sanchez, Jean-CharlesUniv Geneva, Human Prot Sci Dept, Ctr Med Univ, Geneva, Switzerland.
(author)
-
Bergsten, PeterUppsala universitet,Institutionen för medicinsk cellbiologi(Swepub:uu)peteberg
(author)
-
Univ Luxembourg, Luxembourg Ctr Syst Biomed, Esch Belval, Luxembourg.Institutionen för medicinsk cellbiologi
(creator_code:org_t)
Related titles
-
In:PLOS ONE: Public Library of Science (PLoS)12:41932-6203
Internet link
Find in a library
-
PLOS ONE
(Search for host publication in LIBRIS)
To the university's database
- By the author/editor
-
Roomp, Kirsten
-
Kristinsson, Hja ...
-
Schvartz, Domiti ...
-
Ubhayasekera, Ku ...
-
Sargsyan, Ernest
-
Manukyan, Levon
-
show more...
-
Chowdhury, Azazu ...
-
Manell, Hannes
-
Satagopam, Venka ...
-
Groebe, Karlfrie ...
-
Schneider, Reinh ...
-
Bergquist, Jonas
-
Sanchez, Jean-Ch ...
-
Bergsten, Peter
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Endocrinology an ...
- Articles in the publication
-
PLOS ONE
- By the university
-
Uppsala University