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A Combinatorial App...
A Combinatorial Approach to Induce Sensory Axon Regeneration into the Dorsal Root Avulsed Spinal Cord
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- Hoeber, Jan, 1986- (author)
- Uppsala universitet,Regenerativ neurobiologi,Regenerative Neurobiology
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- König, Niclas, 1986- (author)
- Uppsala universitet,Regenerativ neurobiologi,Regenerative Neurobiology
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- Trolle, Carl, 1985- (author)
- Uppsala universitet,Regenerativ neurobiologi,Regenerative Neurobiology
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- Lekholm, Emilia (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Funktionell farmakologi,Molecular Neuropharmacology
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- Zhou, Chunfang (author)
- Nanologica AB , Södertälje, Sweden
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- Pankratova, Stanislava (author)
- Univ Copenhagen, Inst Neurosci & Pharmacol, Copenhagen, Denmark,Institute of Neuroscience and Pharmacology, University of Copenhagen , Copenhagen, Denmark
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- Åkesson, Elisabet (author)
- Karolinska Institutet
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- Fredriksson, Robert (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Molecular Neuropharmacology
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- Aldskogius, Håkan, 1943- (author)
- Uppsala universitet,Regenerativ neurobiologi,Regenerative Neurobiology
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- Kozlova, Elena (author)
- Uppsala universitet,Regenerativ neurobiologi,Regenerative Neurobiology
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(creator_code:org_t)
- Mary Ann Liebert Inc, 2017
- 2017
- English.
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In: Stem Cells and Development. - : Mary Ann Liebert Inc. - 1547-3287 .- 1557-8534. ; 26:14, s. 1065-1077
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Spinal root injuries result in newly formed glial scar formation, which prevents regeneration of sensory axons causing permanent sensory loss. Previous studies showed that delivery of trophic factors or implantation of human neural progenitor cells supports sensory axon regeneration and partly restores sensory functions. In this study, we elucidate mechanisms underlying stem cell-mediated ingrowth of sensory axons after dorsal root avulsion (DRA). We show that human spinal cord neural stem/progenitor cells (hscNSPC), and also, mesoporous silica particles loaded with growth factor mimetics (MesoMIM), supported sensory axon regeneration. However, when hscNSPC and MesoMIM were combined, sensory axon regeneration failed. Morphological and tracing analysis showed that sensory axons grow through the newly established glial scar along "bridges" formed by migrating stem cells. Coimplantation of MesoMIM prevented stem cell migration, "bridges" were not formed, and sensory axons failed to enter the spinal cord. MesoMIM applied alone supported sensory axons ingrowth, but without affecting glial scar formation. In vitro, the presence of MesoMIM significantly impaired migration of hscNSPC without affecting their level of differentiation. Our data show that (1) the ability of stem cells to migrate into the spinal cord and organize cellular "bridges" in the newly formed interface is crucial for successful sensory axon regeneration, (2) trophic factor mimetics delivered by mesoporous silica may be a convenient alternative way to induce sensory axon regeneration, and (3) a combinatorial approach of individually beneficial components is not necessarily additive, but can be counterproductive for axonal growth.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
Keyword
- biomimetics
- neural stem cells
- spinal cord regeneration
- stem cell transplantation
Publication and Content Type
- ref (subject category)
- art (subject category)
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Hoeber, Jan, 198 ...
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König, Niclas, 1 ...
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Trolle, Carl, 19 ...
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Lekholm, Emilia
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Zhou, Chunfang
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Pankratova, Stan ...
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Åkesson, Elisabe ...
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Fredriksson, Rob ...
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Aldskogius, Håka ...
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Kozlova, Elena
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Medical Biotechn ...
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MEDICAL AND HEAL ...
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and Hematology
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Stem Cells and D ...
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Uppsala University
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Karolinska Institutet