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Saline catalyse oxi...
Saline catalyse oxime reaction at physiological pH : overcoming a major limitation of bioorthogonal reaction
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- Wang, Shujiang (author)
- Uppsala universitet,Polymerkemi
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- Nawale, Ganesh N. (author)
- Uppsala universitet,Polymerkemi
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- Kadekar, Sandeep (author)
- Uppsala universitet,Polymerkemi
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- Oommen, Oommen P. (author)
- Tampere University of Technology, and BioMediTech Institute, Finland
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- Jena, Naresh K. (author)
- Uppsala universitet,Materialteori
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- Chakraborty, Sudip (author)
- Uppsala universitet,Materialteori
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- Hilborn, Jöns, 1956- (author)
- Uppsala universitet,Polymerkemi
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- Varghese, Oommen P., 1977- (author)
- Uppsala universitet,Polymerkemi
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(creator_code:org_t)
- English.
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Abstract
Subject headings
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- We have discovered a simple and versatile reaction condition for oxime mediated bioconjugation reaction that could be adapted for both aldehyde and keto substrates. We found that saline accelerated the oxime kinetics in a concentration dependent manner under physiological conditions. The reaction mechanism is validated by computational studies, and the versatility of the reaction is demonstrated by cell-surface labeling experiments. Saline offers an efficient and non-toxic catalytic option for performing the bioorthogonal-coupling reaction of biomolecules at the physiological pH. This saline mediated bioconjugation reaction represents the most bio-friendly, mild and versatile approach for conjugating sensitive biomolecules and does not require any extensive purification step.
Subject headings
- NATURVETENSKAP -- Kemi -- Polymerkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Polymer Chemistry (hsv//eng)
Keyword
- Oxime reaction
- catalysis
- kinetics
- labeling
Publication and Content Type
- ref (subject category)
- art (subject category)
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