Oncolytic alphavirus SFV-VA7 efficiently eradicates subcutaneous and orthotopic human prostate tumours in mice

Martikainen, MiikaUppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Univ Eastern Finland, AI Virtanen Inst Mol Sci, Dept Biotechnol & Mol Med, Kuopio 70211, Finland (author)

Background: Despite recent therapeutic and diagnostic advances, prostate cancer remains the second leading cause of cancer-related deaths among men in the Western world. Oncolytic viruses that replicate selectively in tumour cells represent a novel treatment candidate for these malignancies.Methods: We analysed infectivity of avirulent Semliki Firest virus SFV-VA7 in human prostate cancer cell lines VCaP, LNCaP and 22Rv1 and in nonmalignant prostate epithelial cell line RWPE-1. Therapeutic potency of SFV-VA7 was evaluated in subcutaneous and orthotopic mouse LNCaP xenograft models.Results: SFV-VA7 infected and killed the tested human prostate cancer cell lines irrespective of their hormone response status, while the nonmalignant prostate epithelial cell line RWPE-1 proved highly virus resistant. Notably, a single peritoneal dose of SFV-VA7 was sufficient to eradicate all subcutaneous and orthotopic LNCaP tumours.Conclusions: Our results indicate that SFV-VA7 is a novel, promising therapeutic virus against prostate cancer warranting further testing in early clinical trials.

Ruotsalainen, JanneUniv Eastern Finland, AI Virtanen Inst Mol Sci, Dept Biotechnol & Mol Med, Kuopio 70211, Finland.;Univ Hosp Magdeburg, Dept Dermatol, D-39120 Magdeburg, Germany. (author)
Tuomela, JohannaUniv Turku, Inst Biomed, FIN-20520 Turku, Finland. (author)
Harkonen, PirkkoUniv Turku, Inst Biomed, FIN-20520 Turku, Finland. (author)
Essand, MagnusUppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi(Swepub:uu)magnessa (author)
Heikkila, JariAbo Akad Univ, Dept Biochem & Pharm, Turku 20500, Finland. (author)
Hinkkanen, AriUniv Eastern Finland, AI Virtanen Inst Mol Sci, Dept Biotechnol & Mol Med, Kuopio 70211, Finland. (author)
Uppsala universitetScience for Life Laboratory, SciLifeLab (creator_code:org_t)

In:British Journal of Cancer: NATURE PUBLISHING GROUP117:1, s. 51-550007-09201532-1827

By the author/editor: Martikainen, Mii ...; Ruotsalainen, Ja ...; Tuomela, Johanna; Harkonen, Pirkko; Essand, Magnus; Heikkila, Jari; show more...; Hinkkanen, Ari; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Cancer and Oncol ...

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.