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Effects of sterilization on dissolved organic carbon (DOC) composition and bacterial utilization of DOC from lakes
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- Andersson, Martin G. I. (author)
- Uppsala universitet,Limnologi
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- Catalán, Núria, 1985- (author)
- Uppsala universitet,Limnologi,ICRA, Catalan Institute of Water Research, Girona, Spain
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- Rahman, Zeeshanur (author)
- Uppsala universitet,Limnologi,Applied Microbiology and Biotechnology Laboratory, Department of Botany, University of Delhi
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- (creator_code:org_t)
- Inter-Research Science Center, 2018
- 2018
- English.
- In: Aquatic Microbial Ecology. - : Inter-Research Science Center. - 0948-3055 .- 1616-1564. ; 82:2, s. 199-208
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Sterilization of dissolved organic carbon (DOC) is an essential step in research on interactions between DOC and organisms, for example where the effect of different microbial communities on DOC is studied or vice versa. However, few studies have gone beyond acknowledging that sterilization of DOC influences its characteristics. Here, we aimed to provide further knowledge that enables scientists to better tailor their sterilization methods to their research question. To meet this aim, we conducted a sterilization experiment with DOC from 4 boreal lakes treated with 4 sterilization methods, i.e. 2 filtrations (0.2 µm, 0.1 µm) and 2 autoclaving approaches (single and double autoclaving with a single pH adjustment). Quantity and spectroscopic properties of DOC, before and after sterilization, were studied, and DOC was further tested as a substrate for bacterial growth. We found that the filtration methods better preserved the different DOC measures. In contrast, autoclaving caused major inconsistent shifts in both qualitative and quantitative measures of DOC, as well as an increase of the maximum abundance of bacteria in growth experiments. Nonetheless, there remains a trade-off between retaining the quality of DOC and achieving sterile conditions. Therefore, the sterilization method of choice should be guided by the scientific question at hand.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- sterilization
- autoclave
- filtration
- dissolved organic carbon
- excitation emission matrices
- parallel factor analysis
- Mikrobiologi
- Microbiology
Publication and Content Type
- ref (subject category)
- art (subject category)
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