SwePub
Sök i LIBRIS databas

  Extended search

onr:"swepub:oai:DiVA.org:uu-333703"
 

Search: onr:"swepub:oai:DiVA.org:uu-333703" > Comparison of cellu...

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist
  • Ardesjö-Lundgren, BritaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi,Swedish Univ Agr Sci, Dept Anim Breeding & Genet, Box 7023, SE-75007 Uppsala, Sweden,Institutionen för husdjursgenetik (HGEN),Department of Animal Breeding and Genetics,Uppsala University (author)

Comparison of cellular location and expression of Plakophilin-2 in epidermal cells from nonlesional atopic skin and healthy skin in German shepherd dogs

  • Article/chapterEnglish2017

Publisher, publication year, extent ...

  • 2017-04-06
  • Wiley,2017
  • electronicrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-333703
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-333703URI
  • https://doi.org/10.1111/vde.12441DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:136399495URI
  • https://res.slu.se/id/publ/86717URI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • BackgroundCanine atopic dermatitis (CAD) is an inflammatory and pruritic allergic skin disease caused by interactions between genetic and environmental factors. Previously, a genome‐wide significant risk locus on canine chromosome 27 for CAD was identified in German shepherd dogs (GSDs) and Plakophilin‐2 (PKP2) was defined as the top candidate gene. PKP2 constitutes a crucial component of desmosomes and also is important in signalling, metabolic and transcriptional activities.ObjectivesThe main objective was to evaluate the role of PKP2 in CAD by investigating PKP2 expression and desmosome structure in nonlesional skin from CAD‐affected (carrying the top GWAS SNP risk allele) and healthy GSDs. We also aimed at defining the cell types in the skin that express PKP2 and its intracellular location.Animals/MethodsSkin biopsies were collected from nine CAD‐affected and five control GSDs. The biopsies were frozen for immunofluorescence and fixed for electron microscopy immunolabelling and morphology.ResultsWe observed the novel finding of PKP2 expression in dendritic cells and T cells in dog skin. Moreover, we detected that PKP2 was more evenly expressed within keratinocytes compared to its desmosomal binding‐partner plakoglobin. PKP2 protein was located in the nucleus and on keratin filaments attached to desmosomes. No difference in PKP2 abundance between CAD cases and controls was observed.ConclusionPlakophilin‐2 protein in dog skin is expressed in both epithelial and immune cells; based on its subcellular location its functional role is implicated in both nuclear and structural processes.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Tengvall, Katarina,1980-Karolinska Institutet,Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi,Karolinska Inst, Neuroimmunol Unit, Centrum Mol Med, Dept Clin Neurosci, S-17176 Stockholm, Sweden,Uppsala University(Swepub:slu)49591 (author)
  • Bergvall, KerstinSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences(Swepub:slu)47499 (author)
  • Farias, Fabiana H. G.Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi(Swepub:uu)fabfa647 (author)
  • Wang, LiyaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB)(Swepub:slu)50146 (author)
  • Hedhammar, ÅkeSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences(Swepub:slu)49648 (author)
  • Lindblad-Toh, KerstinUppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi,Broad Inst MIT & Harvard, 415 Main St, Cambridge, MA 02142 USA(Swepub:uu)kerli865 (author)
  • Andersson, GöranSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för husdjursgenetik (HGEN),Department of Animal Breeding and Genetics(Swepub:slu)50422 (author)
  • Uppsala universitetScience for Life Laboratory, SciLifeLab (creator_code:org_t)
  • Sveriges lantbruksuniversitet

Related titles

  • In:Veterinary dermatology (Print): Wiley28:4, s. 377-e880959-44931365-3164

Internet link

Find in a library

To the university's database

  • 1 of 1
  • Previous record
  • Next record
  •    To hitlist

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view