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Enhancement of cilia sub-structures by multiple instance registration and super-resolution reconstruction
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- Suveer, Amit (author)
- Uppsala universitet,Avdelningen för visuell information och interaktion,Bildanalys och människa-datorinteraktion
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- Sladoje, Nataša (author)
- Uppsala universitet,Avdelningen för visuell information och interaktion,Bildanalys och människa-datorinteraktion
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- Lindblad, Joakim (author)
- Uppsala universitet,Avdelningen för visuell information och interaktion,Bildanalys och människa-datorinteraktion
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- (creator_code:org_t)
- 2017-05-19
- 2017
- English.
- In: Image Analysis. - Cham : Springer. - 9783319591285 ; , s. 362-374
- Conference paper (peer-reviewed)
Abstract
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- Ultrastructural analysis of cilia cross-sectional images using transmission electron microscopy (TEM) assists the pathologists to diagnose Primary Ciliary Dyskinesia, a genetic disease. The current diagnostic procedure is manual and difficult because of poor signal-to-noise ratio in TEM images. In this paper, we propose an automated multi-step registration approach to register many cilia cross-sectional instances. The novelty of the work is in the utilization of customized weight masks at each registration step to achieve good alignment of the specific cilium regions. Registration is followed by super-resolution reconstruction to enhance the substructural information. Landmarks matching based evaluation of registration results in pixel alignment error of 2.35±1.82" role="presentation">2.35±1.82 pixels, and the subjective analysis of super-resolution reconstructed cilium shows a clear improvement in the visibility of the substructures such as dynein arms, radial spokes, and central pair.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk bildbehandling (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Image Processing (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
Keyword
- Computerized Image Processing
- Datoriserad bildbehandling
- Patologi
- Pathology
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- kon (subject category)
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