The role of chromatin density in cell population heterogeneity during stem cell differentiation

Golkaram, Mahdi (author)

Jang, Jiwon (author)

Hellander, Stefan (author): Uppsala universitet,Avdelningen för beräkningsvetenskap,Tillämpad beräkningsvetenskap

Kosik, Kenneth S. (author)

Petzold, Linda R. (author)

(creator_code:org_t)

2017-10-17
2017
English.
In: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7, s. 13307:1-11

Related links:: https://doi.org/10.1...; show more...; https://www.nature.c...; https://urn.kb.se/re...; https://doi.org/10.1...; show less...

Abstract Subject headings

We incorporate three-dimensional (3D) conformation of chromosome (Hi-C) and single-cell RNA sequencing data together with discrete stochastic simulation, to explore the role of chromatin reorganization in determining gene expression heterogeneity during development. While previous research has emphasized the importance of chromatin architecture on activation and suppression of certain regulatory genes and gene networks, our study demonstrates how chromatin remodeling can dictate gene expression distribution by folding into distinct topological domains. We hypothesize that the local DNA density during differentiation accentuate transcriptional bursting due to the crowding effect of chromatin. This phenomenon yields a heterogeneous cell population, thereby increasing the potential of differentiation of the stem cells.

By the author/editor: Golkaram, Mahdi; Jang, Jiwon; Hellander, Stefa ...; Kosik, Kenneth S ...; Petzold, Linda R ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Computer and Inf ...; and Bioinformatics

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