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Nanosecond pump-pro...
Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA
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- Kubo, Minoru (author)
- RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan.;Japan Sci & Technol Agcy, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 3320012, Japan.
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- Nango, Eriko (author)
- RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan.;Kyoto Univ, Grad Sch Med, Dept Cell Biol, Sakyo Ku, Yoshidakonoe Cho, Kyoto 6068501, Japan.
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- Tono, Kensuke (author)
- Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan.
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- Kimura, Tetsunari (author)
- RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan.;Kobe Univ, Grad Sch Sci, Dept Chem, Nada Ku, 1-1 Rokkodai, Kobe, Hyogo 6578501, Japan.
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- Owada, Shigeki (author)
- RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan.
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- Song, Changyong (author)
- RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan.;Pohang Univ Sci & Technol, Dept Phys, Pohang 790784, South Korea.
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- Mafune, Fumitaka (author)
- Univ Tokyo, Sch Arts & Sci, Dept Basic Sci, Meguro Ku, Tokyo 1538902, Japan.
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- Miyajima, Ken (author)
- Univ Tokyo, Sch Arts & Sci, Dept Basic Sci, Meguro Ku, Tokyo 1538902, Japan.
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- Takeda, Yoshihiro (author)
- Genesis Res Inst Inc, East Tokyo Lab, Ichikawa, Chiba 2720001, Japan.
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- Kohno, Jun-ya (author)
- Gakushuin Univ, Sch Sci, Dept Chem, Toshima Ku, Tokyo, Japan.
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- Miyauchi, Naoya (author)
- Natl Inst Mat Sci, Res Ctr Adv Measurement & Characterizat, Tsukuba, Ibaraki 3050047, Japan.
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- Nakane, Takanori (author)
- Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1130033, Japan.
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- Tanaka, Tomoyuki (author)
- RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan.
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- Nomura, Takashi (author)
- RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan.
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- Davidsson, Jan (author)
- Uppsala universitet,Fysikalisk kemi
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- Tanaka, Rie (author)
- RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan.
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- Murata, Michio (author)
- Osaka Univ, Lipid Act Struct Project, JST ERATO, 1-1 Machikaneyama, Toyonaka, Osaka 5600043, Japan.
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- Kameshima, Takashi (author)
- Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan.
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- Hatsui, Takaki (author)
- RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan.
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- Joti, Yasumasa (author)
- Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan.
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- Neutze, Richard (author)
- Univ Gothenburg, Dept Chem & Mol Biol, Box 462, SE-40530 Gothenburg, Sweden.
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- Yabashi, Makina (author)
- RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan.
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- Iwata, So (author)
- RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan.;Kyoto Univ, Grad Sch Med, Dept Cell Biol, Sakyo Ku, Yoshidakonoe Cho, Kyoto 6068501, Japan.
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RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan;Japan Sci & Technol Agcy, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 3320012, Japan. RIKEN SPring 8 Ctr, 1-1-1 Kouto, Sayo, Hyogo 6795148, Japan.;Kyoto Univ, Grad Sch Med, Dept Cell Biol, Sakyo Ku, Yoshidakonoe Cho, Kyoto 6068501, Japan. (creator_code:org_t)
- INT UNION CRYSTALLOGRAPHY, 2017
- 2017
- English.
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In: Journal of Synchrotron Radiation. - : INT UNION CRYSTALLOGRAPHY. - 0909-0495 .- 1600-5775. ; 24, s. 1086-1091
- Related links:
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
Close
- X-ray free-electron lasers (XFELs) have opened new opportunities for timeresolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TRSFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.
Subject headings
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
Keyword
- XFEL
- serial femtosecond crystallography
- time-resolved X-ray crystallography
- pump and probe
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Kubo, Minoru
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Nango, Eriko
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Tono, Kensuke
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Kimura, Tetsunar ...
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Owada, Shigeki
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Song, Changyong
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show more...
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Mafune, Fumitaka
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Miyajima, Ken
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Takeda, Yoshihir ...
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Kohno, Jun-ya
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Miyauchi, Naoya
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Nakane, Takanori
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Tanaka, Tomoyuki
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Nomura, Takashi
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Davidsson, Jan
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Tanaka, Rie
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Murata, Michio
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Kameshima, Takas ...
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Hatsui, Takaki
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Joti, Yasumasa
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Neutze, Richard
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Yabashi, Makina
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Iwata, So
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Atom and Molecul ...
- Articles in the publication
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Journal of Synch ...
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Uppsala University