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PP2A-B' holoenzyme ...
PP2A-B' holoenzyme substrate recognition, regulation and role in cytokinesis
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- Wu, Cheng-Guo (author)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA.; Univ Wisconsin Madison, Biophys Program, Madison, WI 53706 USA.; DuPont Ind Biosci, Genencor Div, 925 Page Mill Rd, Palo Alto, CA 94304 USA
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- Chen, Hui (author)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA.; DuPont Ind Biosci, Genencor Div, 925 Page Mill Rd, Palo Alto, CA 94304 USA
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- Guo, Feng (author)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA.; DuPont Ind Biosci, Genencor Div, 925 Page Mill Rd, Palo Alto, CA 94304 USA.; Univ Pittsburgh, Dept Microbiol & Mol Genet, Pittsburgh, PA USA
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- Yadav, Vikash K (author)
- Uppsala universitet,Institutionen för kemi - BMC,DuPont Ind Biosci, Genencor Div, 925 Page Mill Rd, Palo Alto, CA 94304 USA,Ivarsson
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- Mcilwain, Sean J (author)
- Univ Wisconsin Madison, Sch Med & Publ Hlth, Biostat & Med Informat, Wisconsin Inst Med Res, Madison, WI USA
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- Rowse, Michael (author)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA
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- Choudhary, Alka (author)
- Univ Wisconsin Madison, Sch Med & Publ Hlth, Dept Med, UW Carbone Canc Ctr,Hematol Oncol, Madison, WI USA
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- Lin, Ziqing (author)
- Sch Med & Publ Hlth, Dept Cell & Regenerat Biol, Human Prote Program, Madison, WI USA
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- Li, Yitong (author)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA
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- Gu, Tingjia (author)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA
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- Zheng, Aiping (author)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA.; Univ Pittsburgh, Dept Microbiol & Mol Genet, Pittsburgh, PA USA
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- Xu, Qingge (author)
- Sch Med & Publ Hlth, Dept Cell & Regenerat Biol, Human Prote Program, Madison, WI USA
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- Lee, Woojong (author)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA
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- Resch, Eduard (author)
- Fraunhofer Inst Mol Biol & Appl Ecol IME, Project Grp Translat Med & Pharmacol TMP, Frankfurt, Germany
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- Johnson, Benjamin (author)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA
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- Day, Jenny (author)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA
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- Ge, Ying (author)
- Sch Med & Publ Hlth, Dept Cell & Regenerat Biol, Human Prote Program, Madison, WI USA
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- Ong, Irene M (author)
- Univ Wisconsin Madison, Sch Med & Publ Hlth, Biostat & Med Informat, Wisconsin Inst Med Res, Madison, WI USA
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- Burkard, Mark E (author)
- Univ Wisconsin Madison, Sch Med & Publ Hlth, Dept Med, UW Carbone Canc Ctr,Hematol Oncol, Madison, WI USA
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- Ivarsson, Ylva (author)
- Uppsala universitet,Biokemi,Ivarsson
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- Xing, Yongna (author)
- Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA.; Univ Wisconsin Madison, Biophys Program, Madison, WI 53706 USA
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Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA; Univ Wisconsin Madison, Biophys Program, Madison, WI 53706 USA.; DuPont Ind Biosci, Genencor Div, 925 Page Mill Rd, Palo Alto, CA 94304 USA Univ Wisconsin Madison, McArdle Lab Canc Res, Dept Oncol, Sch Med & Publ Hlth, Madison, WI 53706 USA.; DuPont Ind Biosci, Genencor Div, 925 Page Mill Rd, Palo Alto, CA 94304 USA (creator_code:org_t)
- 2017-08-08
- 2017
- English.
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In: Cell Discovery. - : Springer Science and Business Media LLC. - 2056-5968. ; 3
- Related links:
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Abstract
Subject headings
Close
- Protein phosphatase 2A (PP2A) is a major Ser/Thr phosphatase; it forms diverse heterotrimeric holoenzymes that counteract kinase actions. Using a peptidome that tiles the disordered regions of the human proteome, we identified proteins containing [LMFI]xx[ILV]xEx motifs that serve as interaction sites for B'-family PP2A regulatory subunits and holoenzymes. The B'-binding motifs have important roles in substrate recognition and in competitive inhibition of substrate binding. With more than 100 novel ligands identified, we confirmed that the recently identified LxxIxEx B'α-binding motifs serve as common binding sites for B' subunits with minor variations, and that S/T phosphorylation or D/E residues at positions 2, 7, 8 and 9 of the motifs reinforce interactions. Hundreds of proteins in the human proteome harbor intrinsic or phosphorylation-responsive B'-interaction motifs, and localize at distinct cellular organelles, such as midbody, predicting kinase-facilitated recruitment of PP2A-B' holoenzymes for tight spatiotemporal control of phosphorylation at mitosis and cytokinesis. Moroever, Polo-like kinase 1-mediated phosphorylation of Cyk4/RACGAP1, a centralspindlin component at the midbody, facilitates binding of both RhoA guanine nucleotide exchange factor (epithelial cell transforming sequence 2 (Ect2)) and PP2A-B' that in turn dephosphorylates Cyk4 and disrupts Ect2 binding. This feedback signaling loop precisely controls RhoA activation and specifies a restricted region for cleavage furrow ingression. Our results provide a framework for further investigation of diverse signaling circuits formed by PP2A-B' holoenzymes in various cellular processes.
Subject headings
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Keyword
- CIP2A
- PP2A-B′ holoenzyme
- SLiMs
- centrosome
- cytokinesis
- midbody
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
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Wu, Cheng-Guo
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Chen, Hui
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Guo, Feng
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Yadav, Vikash K
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Mcilwain, Sean J
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Rowse, Michael
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show more...
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Choudhary, Alka
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Lin, Ziqing
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Li, Yitong
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Gu, Tingjia
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Zheng, Aiping
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Xu, Qingge
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Lee, Woojong
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Resch, Eduard
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Johnson, Benjami ...
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Day, Jenny
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Ge, Ying
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Ong, Irene M
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Burkard, Mark E
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Ivarsson, Ylva
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Xing, Yongna
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Bioinformatics a ...
- Articles in the publication
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Cell Discovery
- By the university
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Uppsala University