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Multicenter Assessm...
Multicenter Assessment of Animal-free Collagenase AF-1 for Human Islet Isolation
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- Brandhorst, Daniel (author)
- Univ Oxford, Nuffield Dept Surg Sci, Oxford, England.;Churchill Hosp, OCDEM, Oxford, England.
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- Parnaud, Geraldine (author)
- Geneva Univ Hosp, Dept Surg, Cell Isolat & Transplantat Ctr, Geneva, Switzerland.
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- Friberg, Andrew, 1979- (author)
- Uppsala universitet,Klinisk immunologi
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- Lavallard, Vanessa (author)
- Geneva Univ Hosp, Dept Surg, Cell Isolat & Transplantat Ctr, Geneva, Switzerland.
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- Demuylder-Mischler, Sandrine (author)
- Geneva Univ Hosp, Dept Surg, Cell Isolat & Transplantat Ctr, Geneva, Switzerland.
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- Hughes, Stephen (author)
- Univ Oxford, Nuffield Dept Surg Sci, Oxford, England.;Churchill Hosp, OCDEM, Oxford, England.
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- Saphoerster, Julia (author)
- SERVA Electrophoresis GmbH, Uetersen, Germany.
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- Kurfuerst, Manfred (author)
- SERVA Electrophoresis GmbH, Uetersen, Germany.
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- Korsgren, Olle (author)
- Uppsala universitet,Klinisk immunologi
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- Berney, Thierry (author)
- Geneva Univ Hosp, Dept Surg, Cell Isolat & Transplantat Ctr, Geneva, Switzerland.
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- Johnson, Paul R. V. (author)
- Univ Oxford, Nuffield Dept Surg Sci, Oxford, England.;Churchill Hosp, OCDEM, Oxford, England.
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Univ Oxford, Nuffield Dept Surg Sci, Oxford, England;Churchill Hosp, OCDEM, Oxford, England. Geneva Univ Hosp, Dept Surg, Cell Isolat & Transplantat Ctr, Geneva, Switzerland. (creator_code:org_t)
- 2017-12-18
- 2017
- English.
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In: Cell Transplantation. - : Sage Publications. - 0963-6897 .- 1555-3892. ; 26:10, s. 1688-1693
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Abstract
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- Animal-free (AF) SERVA Collagenase AF-1 and Neutral Protease (NP) AF GMP Grade have recently become available for human islet isolation. This report describes the initial experiences of 3 different islet transplant centers. Thirty-four human pancreases were digested using 1 vial of the 6 different lots of Collagenase AF-1 (2,000-2,583 PZ-U/vial) supplemented with 4 different lots of NP AF in a range of 50 to 160 DMC-U per pancreas. Isolation, culture, and quality assessment were performed using standard techniques as previously described. All data are presented as mean +/- standard error of the mean (SEM). Variability of pancreas weight was associated with a wide range of collagenase and NP activities, ranging from 12.7 to 46.6 PZ-U/g (26.0 +/- 1.5 PZ-U/g) and 0.4 to 3.0 DMC-U/g (1.5 +/- 0.1 DMC-U/g), respectively. Postpurification islet yield was 296,494 +/- 33,620 islet equivalents (IEQ) equivalent to 3,274 +/- 450 IEQ/g with a purity of 55.9% +/- 3.2%. Quality assessment performed after 2 to 4 d of culture demonstrated a viability of 88.1% +/- 1.5% and a stimulation index of 3.7 +/- 0.7. Eighteen of the 34 preparations were transplanted into type 1 diabetic patients equivalent to a transplantation rate of 52.9%. Six preparations, which were infused into patients as first transplant, could be analyzed and increased the fasting C-peptide level from 0.11 +/- 0.08 pretransplant to 1.23 +/- 0.24 and 2.27 +/- 0.31 ng/mL 3 and 6 mo posttransplant (P < 0.05), respectively. Insulin requirements were simultaneously reduced at the same time from 39.2 +/- 3.8 IU/d before transplantation to 10.8 +/- 4.1 and 4.0 +/- 2.3 IU/d, after 3 and 6 mo posttransplant (P < 0.05), respectively. This study demonstrates the efficiency of AF SERVA Collagenase AF-1 and NP AF for clinical islet isolation and transplantation. The new plant-based production process makes these products a safe new option for the islet field.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Keyword
- human islet isolation
- human islet transplantation
- collagenase
- islet quality assessment
Publication and Content Type
- ref (subject category)
- art (subject category)
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Brandhorst, Dani ...
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Parnaud, Geraldi ...
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Friberg, Andrew, ...
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Lavallard, Vanes ...
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Demuylder-Mischl ...
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Hughes, Stephen
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Saphoerster, Jul ...
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Kurfuerst, Manfr ...
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Korsgren, Olle
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Berney, Thierry
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Johnson, Paul R. ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Endocrinology an ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
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Cell Transplanta ...
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Uppsala University