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Mast cell chymase d...
Mast cell chymase decreases the severity of group B Streptococcus infections
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Gendrin, Claire (author)
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Shubin, Nicholas J (author)
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Boldenow, Erica (author)
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Merillat, Sean (author)
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Clauson, Morgan (author)
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Power, Danial (author)
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Doran, Kelly S (author)
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- Åbrink, Magnus (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health
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- Pejler, Gunnar (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Department of Anatomy, Physiology and Biochemistry, the Swedish University of Agricultural Sciences, Uppsala, Sweden,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB),Uppsala University
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Rajagopal, Lakshmi (author)
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Piliponsky, Adrian M (author)
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(creator_code:org_t)
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- Elsevier BV, 2018
- 2018
- English.
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In: Journal of Allergy and Clinical Immunology. - : Elsevier BV. - 0091-6749 .- 1097-6825. ; 142:1, s. 120-129
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http://www.jacionlin...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- BACKGROUND: Group B Streptococcus (GBS) or Streptococcus agalactiae are β-hemolytic gram-positive bacteria that colonize the lower genital tracts of women and are frequently associated with infections during pregnancy. Innate immune defenses are critical for controlling GBS dissemination and systemic infection. Mast cells are resident sentinel cells that come into contact with pathogens early during colonization and infection.OBJECTIVE: We aimed to investigate the contribution of chymase to systemic GBS infection and rates of preterm birth.METHODS: Pharmacologic and genetic approaches using mice deficient in mast cell protease (MCPT) 4, the mouse functional homologue of human chymase, were used.RESULTS: Our studies show that mast cells release a protease with chymotrypsin-like cleavage specificity in response to GBS. Additionally, increased GBS systemic infection and preterm births were observed in MCPT4-deficient mice versus MCPT4-sufficient mice. Furthermore, we observed that proteolytic cleavage of the host extracellular matrix protein fibronectin by peritoneal cell-derived mast cell lysates diminished GBS adherence. Consistent with this observation, the increase in GBS dissemination and preterm births observed in MCPT4-deficient mice was abolished when GBS was deficient in expression of the fibronectin-binding protein SfbA.CONCLUSIONS: Taken together, our results suggest that the protective effect of MCPT4 against GBS dissemination and preterm labor can be attributed in part to MCPT4-mediated proteolysis of fibronectin. Our studies reveal a novel role of mast cells in defense against bacterial infections.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Keyword
- Group B Streptococcus
- chymase
- fibronectin
- mast cells
- mouse mast cell protease 4
- proteases
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Gendrin, Claire
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Shubin, Nicholas ...
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Boldenow, Erica
-
Merillat, Sean
-
Clauson, Morgan
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Power, Danial
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show more...
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Doran, Kelly S
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Åbrink, Magnus
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Pejler, Gunnar
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Rajagopal, Laksh ...
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Piliponsky, Adri ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Immunology
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Uppsala University
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Swedish University of Agricultural Sciences