onr:"swepub:oai:DiVA.org:uu-342430"
Search: onr:"swepub:oai:DiVA.org:uu-342430" > Evaluation of Post-...
- 1 of 1
- Previous record
- Next record
- To hitlist
- Sjöholm, Louise KKarolinska Institutet (author)
Evaluation of Post-Mortem Effects on Global Brain DNA Methylation and Hydroxymethylation.
- Article/chapterEnglish2018
Publisher, publication year, extent ...
- 2017-11-13
- Wiley,2018
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-342430
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-342430URI
- https://doi.org/10.1111/bcpt.12875DOI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:137964734URI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- The number of epigenetic studies on brain functions and diseases are dramatically increasing, but little is known about the impact of post-mortem intervals and post-sampling effects on DNA modifications such as 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). Here, we examined post-mortem-induced changes in global brain 5mC and 5hmC levels at post-mortem intervals up to 540 min., and studied effects of tissue heat stabilization, using LUMA and ELISA. The global 5mC and 5hmC levels were generally higher in the cerebellum of adult rats than neonates. When measured by ELISA, the global 5mC content in adults, but not neonates, decreased with the post-mortem interval reaching a significantly lower level in cerebellum tissue at the post-mortem interval 540 min. (2.9 ± 0.7%; mean ± S.E.M.) compared to control (3.7 ± 0.6%). The global 5hmC levels increased with post-mortem interval reaching a significantly higher level at 540 min. (0.29 ± 0.06%) compared to control (0.19 ± 0.03%). This suggests that the post-mortem interval may confound 5mC and 5hmC analysis in human brain tissues as the post-mortem handling could vary substantially. The reactive oxygen species (ROS) level in cerebellum also increased over time, in particular in adults, and may be part of the mechanism that causes the observed post-mortem changes in 5mC and 5hmC. The global 5mC and 5hmC states were unaffected by heat stabilization, allowing analysis of tissues that are stabilized to preserve more labile analytes. Further studies in human samples are needed to confirm post-mortem effects on DNA methylation/hydroxymethylation and elucidate details of the underlying mechanisms.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
- Ransome, YusufHarvard T.H. Chan School of Public Health, Boston, USA (author)
- Ekström, Tomas JKarolinska Institutet (author)
- Karlsson, OskarUppsala universitet,Institutionen för farmaceutisk biovetenskap(Swepub:uu)oskar255 (author)
- Karolinska InstitutetHarvard T.H. Chan School of Public Health, Boston, USA (creator_code:org_t)
Related titles
- In:Basic & Clinical Pharmacology & Toxicology: Wiley122:2, s. 208-2131742-78351742-7843
Internet link
Find in a library
- Basic & Clinical Pharmacology & Toxicology (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Sjöholm, Louise ...
- Ransome, Yusuf
- Ekström, Tomas J
- Karlsson, Oskar
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Neurosciences
- Articles in the publication
- Basic & Clinical ...
- By the university
- Uppsala University
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
