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Metabolite aberrati...
Metabolite aberrations in early diabetes detected in rat kidney using mass spectrometry imaging
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- Bergman, Hilde-Marlene (author)
- Uppsala universitet,Analytisk kemi
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- Lindfors, Lina (author)
- Uppsala universitet,Organisk kemi
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- Palm, Fredrik, 1973- (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Kihlberg, Jan (author)
- Uppsala universitet,Organisk kemi
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- Lanekoff, Ingela, Assoc. Prof. 1975- (author)
- Uppsala universitet,Analytisk kemi
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(creator_code:org_t)
- 2019-03-20
- 2019
- English.
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In: Analytical and Bioanalytical Chemistry. - : Springer Science and Business Media LLC. - 1618-2642 .- 1618-2650. ; 411:13, s. 2809-2816
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Abstract
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- Diabetic kidney disease is a serious complication of diabetes that can ultimately lead to end-stage renal disease. The pathogenesis of diabetic kidney disease is complex, and fundamental research is still required to provide a better understanding of the driving forces behind it. We report regional metabolic aberrations from an untargeted mass spectrometry imaging study of kidney tissue using an insulinopenic rat model of diabetes. Diabetes was induced by intravenous injection of streptozotocin, and kidneys were harvested 2weeks thereafter. Imaging was performed using nanospray desorption electrospray ionization connected to a high-mass-resolving mass spectrometer. No histopathological changes were observed in the kidney sections; however, mass spectrometry imaging revealed a significant increase in several 18-carbon unsaturated non-esterified fatty acid species and monoacylglycerols. Notably, these 18-carbon acyl chains were also constituents of several increased diacylglycerol species. In addition, a number of short- and long-chain acylcarnitines were found to be accumulated while several amino acids were depleted. This study presents unique regional metabolic data indicating a dysregulated energy metabolism in renal mitochondria as an early response to streptozotocin-induced type I diabetes.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- Kemi med inriktning mot analytisk kemi
- Chemistry with specialization in Analytical Chemistry
Publication and Content Type
- ref (subject category)
- art (subject category)
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