Search: onr:"swepub:oai:DiVA.org:uu-349997" >
Identification of b...
Identification of binding sites on the regulatory A subunit of protein phosphatase 2A for the catalytic C subunit and for tumor antigens of simian virus 40 and polyomavirus.
- Article/chapterEnglish1992
Publisher, publication year, extent ...
Numbers
-
LIBRIS-ID:oai:DiVA.org:uu-349997
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-349997URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Protein phosphatase 2A is composed of three subunits: the catalytic subunit C and two regulatory subunits, A and B. The A subunit consists of 15 nonidentical repeats and has a rodlike shape. It is associated with the B and C subunits as well as with the simian virus 40 small T, polyomavirus small T, and polyomavirus medium T tumor antigens. We determined the binding sites on subunit A for subunit C and tumor antigens by site-directed mutagenesis of A. Twenty-four N- and C-terminal truncations and internal deletions of A were assayed by coimmunoprecipitation for their ability to bind C and tumor antigens. It was found that C binds to repeats 11 to 15 at the C terminus of A, whereas T antigens bind to overlapping but distinct regions of the N terminus. Simian virus 40 small T binds to repeats 3 to 6, and polyomavirus small T and medium T bind to repeats 2 to 8. The data suggest cooperativity between C and T antigens in binding to A. This is most apparent for medium T antigen, which can only bind to those A subunit molecules that provide the entire binding region for the C subunit. We infer from our results that B also binds to N-terminal repeats. A model of the small T/medium T/B-A-C complexes is presented.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Roeckel, D
(author)
-
Fait, J
(author)
-
Bergqvist, AndersUppsala universitet,Institutionen för medicinska vetenskaper,Institutionen för medicinsk biokemi och mikrobiologi(Swepub:uu)abe14202
(author)
-
Magnusson, G
(author)
-
Walter, G
(author)
-
Uppsala universitetInstitutionen för medicinska vetenskaper
(creator_code:org_t)
Related titles
-
In:Molecular and Cellular Biology12:11, s. 4872-820270-73061098-5549
Internet link
Find in a library
To the university's database