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Non-invasive tri-mo...
Non-invasive tri-modal visualisation via PET/SPECT/μCT of recombinant human bone morphogenetic protein-2 retention and associated bone regeneration : A proof of concept
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- Hulsart Billström, Gry, 1982- (författare)
- Uppsala universitet,Ortopedi
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- Selvaraju, Ramkumar (författare)
- Uppsala universitet,Plattformen för Preklinisk PET-MRI
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- Estrada, Sergio (författare)
- Uppsala universitet,Plattformen för Preklinisk PET-MRI
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- Lubberink, Mark (författare)
- Uppsala universitet,Radiologi
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- Asplund, Veronika (författare)
- Uppsala universitet,Institutionen för läkemedelskemi
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- Bergman, Kristoffer (författare)
- TERMIRA, Stockholm, Sweden
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- Marsell, Richard (författare)
- Uppsala universitet,Ortopedi
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- Larsson, Sune (författare)
- Uppsala universitet,Ortopedi
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- Antoni, Gunnar (författare)
- Uppsala universitet,Preparativ läkemedelskemi
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(creator_code:org_t)
- Elsevier BV, 2018
- 2018
- Engelska.
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Ingår i: Journal of Controlled Release. - : Elsevier BV. - 0168-3659 .- 1873-4995. ; 285, s. 178-186
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Bone morphogenetic proteins (BMP's) are vital for bone and cartilage formation, where bone morphogenetic protein-2 (BMP-2) is acknowledged as a growth factor in osteoblast differentiation. However, uncontrolled delivery may result in adverse clinical effects. In this study we investigated the possibility for longitudinal and non-invasive monitoring of implanted [125I]BMP-2 retention and its relation to ossification at the site of implantation. A unilateral critically sized femoral defect was produced in the left limb of rats while the right femur was retained intact as a paired reference control. The defect was filled with a hyaluronan hydrogel with 25% hydroxyapatite alone (carrier control; n = 2) or combined with a mixture of [125I]BMP-2 (150 μg/ml; n = 4). Bone formation was monitored using micro computed tomography (μCT) scans at 1, 3, 5, 7, 9 and 12 weeks. The retention of [125I]BMP-2 was assessed with single photon emission computed tomography (SPECT), and the bone healing process was followed with sodium fluoride (Na18F) using positron emission tomography (PET) at day 3 and at week 2, 4, and 6. A rapid burst release of [125I]BMP-2 was detected via SPECT. This was followed by a progressive increase in uptake levels of [18F]fluoride depicted by PET imaging that was confirmed as bone formation via μCT. We propose that this functional, non-invasive imaging method allows tri-modal visualisation of the release of BMP-2 and the following in vivo response. We suggest that the potential of this novel technique could be considered for preclinical evaluation of novel smart materials on bone regeneration.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Radiologi och bildbehandling (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Radiology, Nuclear Medicine and Medical Imaging (hsv//eng)
Nyckelord
- Bone morphogenetic protein 2
- Bone tissue engineering
- Hydrogel
- Micro computed tomography
- Positron emission tomography
- Single-photon emission computed tomography
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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