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Expression of trans...
Expression of trans-membrane serine protease 3 (TMPRSS3) in the human organ of Corti
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- Liu, Wei (author)
- Uppsala universitet,Öron-, näs- och halssjukdomar
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- Loewenheim, Hubert (author)
- Eberhard Karls Univ Tubingen, Tubingen Hearing Res Ctr, Dept Otolaryngol Head & Neck Surg, D-72076 Tubingen, Germany
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- Santi, Peter A. (author)
- Univ Minnesota, Dept Otolaryngol, 121 Lions Res Bldg,2001 Sixth St SE, Minneapolis, MN 55455 USA
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- Glueckert, Rudolf (author)
- Med Univ Innsbruck, Dept Otolaryngol, Anichstr 35, A-6020 Innsbruck, Austria
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- Schrott-Fischer, Annelies (author)
- Med Univ Innsbruck, Dept Otolaryngol, Anichstr 35, A-6020 Innsbruck, Austria
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- Rask-Andersen, Helge (author)
- Uppsala universitet,Öron-, näs- och halssjukdomar
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(creator_code:org_t)
- 2018-02-19
- 2018
- English.
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In: Cell and Tissue Research. - : Springer Science and Business Media LLC. - 0302-766X .- 1432-0878. ; 372:3, s. 445-456
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Abstract
Subject headings
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- TMPRSS3 (Trans-membrane Serine Protease 3) is a type II trans-membrane serine protease that has proteolytic activity essential for hearing. Mutations in the gene cause non-syndromic autosomal recessive deafness (DFNB8/10) in humans. Knowledge about its cellular distribution in the human inner ear may increase our understanding of its physiological role and involvement in deafness, ultimately leading to therapeutic interventions. In this study, we used super-resolution structured illumination microscopy for the first time together with transmission electron microscopy to localize the TMPRSS3 protein in the human organ of Corti. Archival human cochleae were dissected out during petroclival meningioma surgery. Microscopy with Zeiss LSM710 microscope achieved a lateral resolution of approximately 80 nm. TMPRSS3 was found to be associated with actin in both inner and outer hair cells. TMPRSS3 was located in cell surface-associated cytoskeletal bodies (surfoskelosomes) in inner and outer pillar cells and Deiters cells and in subcuticular organelles in outer hair cells. Our results suggest that TMPRSS3 proteolysis is linked to hair cell sterociliary mechanics and to the actin/microtubule networks that support cell motility and integrity.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Keyword
- Cochlea
- Trans-membrane Serine Protease 3 (TMPRSS3)
- Immunohistochemistry
- Super-resolution structured illumination microscopy (SR-SIM)
- Human
Publication and Content Type
- ref (subject category)
- art (subject category)
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