Search: onr:"swepub:oai:DiVA.org:uu-357006" >
Prolyl carboxypepti...
Prolyl carboxypeptidase activity in the circulation and its correlation with body weight and adipose tissue in lean and obese subjects
-
- Kehoe, Kaat (author)
- Univ Antwerp, Dept Pharmaceut Sci, Lab Med Biochem, Antwerp, Belgium
-
- Noels, Heidi (author)
- Rhein Westfal TH Aachen, Inst Mol Cardiovasc Res, Aachen, Germany
-
- Theelen, Wendy (author)
- Rhein Westfal TH Aachen, Inst Mol Cardiovasc Res, Aachen, Germany
-
show more...
-
- De Hert, Emilie (author)
- Univ Antwerp, Dept Pharmaceut Sci, Lab Med Biochem, Antwerp, Belgium
-
- Xu, Shenguan (author)
- Uppsala universitet,Klinisk kemi
-
- Verrijken, An (author)
- Antwerp Univ Hosp, Dept Endocrinol Diabetol & Metab, Edegem, Belgium;Univ Antwerp, Fac Med & Hlth Sci, LEMP, Antwerp, Belgium
-
- Arnould, Thierry (author)
- Univ Namur UNamur, Namur Res Inst Life Sci NARILIS, Lab Biochem & Cell Biol URBC, Namur, Belgium
-
- Fransen, Erik (author)
- Univ Antwerp, StatUa Ctr Stat, Antwerp, Belgium
-
- Hermans, Nina (author)
- Univ Antwerp, Dept Pharmaceut Sci, Nat Prod & Food Res & Anal Nat, Antwerp, Belgium
-
- Lambeir, Anne-Marie (author)
- Univ Antwerp, Dept Pharmaceut Sci, Lab Med Biochem, Antwerp, Belgium
-
- Venge, Per (author)
- Uppsala universitet,Klinisk kemi
-
- Van Gaal, Luc (author)
- Antwerp Univ Hosp, Dept Endocrinol Diabetol & Metab, Edegem, Belgium;Univ Antwerp, Fac Med & Hlth Sci, LEMP, Antwerp, Belgium
-
- De Meester, Ingrid (author)
- Univ Antwerp, Dept Pharmaceut Sci, Lab Med Biochem, Antwerp, Belgium
-
show less...
-
(creator_code:org_t)
- 2018-05-17
- 2018
- English.
-
In: PLOS ONE. - : PUBLIC LIBRARY SCIENCE. - 1932-6203. ; 13:5
- Related links:
-
https://doi.org/10.1...
-
show more...
-
https://uu.diva-port... (primary) (Raw object)
-
https://journals.plo...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Background Prolyl carboxypeptidase (PRCP) is involved in the regulation of body weight, likely by hydrolysing alpha-melanocyte-stimulating hormone and apelin in the hypothalamus and in the periphery. A link between PRCP protein concentrations in plasma and metabolic disorders has been reported. In this study, we investigated the distribution of circulating PRCP activity and assessed its relation with body weight and adipose tissue in obese patients and patients who significantly lost weight. Methods PRCP activity was measured using reversed-phase high-performance liquid chromatography in different isolated blood fractions and primary human cells to investigate the distribution of circulating PRCP. PRCP activity was measured in serum of individuals (n = 75) categorized based on their body mass index (BMI < 25.0; 25.0-29.9; 30.0-39.9; >= 40.0 kg/m(2)) and the diagnosis of metabolic syndrome. Differences in serum PRCP activity were determined before and six months after weight loss, either by diet (n = 45) or by bariatric surgery (n = 24). Potential correlations between serum PRCP activity and several metabolic and biochemical parameters were assessed. Additionally, plasma PRCP concentrations were quantified using a sensitive ELISA in the bariatric surgery group. Results White blood cells and plasma contributed the most to circulating PRCP activity. Serum PRCP activity in lean subjects was 0.83 +/- 0.04 U/L and increased significantly with a rising BMI (p<0.001) and decreased upon weight loss (diet, p<0.05; bariatric surgery, p<0.001). The serum PRCP activity alteration reflected body weight changes and was found to be positively correlated with several metabolic parameters, including: total, abdominal and visceral adipose tissue. Plasma PRCP concentration was found to be significantly correlated to serum PRCP activity (0.865; p<0.001). Additionally, a significant decrease (p<0.001) in plasma PRCP protein concentration (mean +/- SD) before (18.2 +/- 3.7 ng/mL) and 6 months after bariatric surgery (15.7 +/- 2.7 ng/mL) was found. Conclusion Our novel findings demonstrate that white blood cells and plasma contributed the most to circulating PRCP activity. Additionally, we have shown that there were significant correlations between serum PRCP activity and various metabolic parameters, and that plasma PRCP concentration was significantly correlated to serum PRCP activity. These novel findings on PRCP activity in serum support further investigation of its in vivo role and involvement in several metabolic diseases.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
PLOS ONE
(Search for host publication in LIBRIS)
To the university's database
- By the author/editor
-
Kehoe, Kaat
-
Noels, Heidi
-
Theelen, Wendy
-
De Hert, Emilie
-
Xu, Shenguan
-
Verrijken, An
-
show more...
-
Arnould, Thierry
-
Fransen, Erik
-
Hermans, Nina
-
Lambeir, Anne-Ma ...
-
Venge, Per
-
Van Gaal, Luc
-
De Meester, Ingr ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Endocrinology an ...
- Articles in the publication
-
PLOS ONE
- By the university
-
Uppsala University