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- Campos-Beltran, DianaUniv Lubeck, Inst Expt & Clin Pharmacol & Toxicol, Ratzeburger Allee 160, D-23562 Lubeck, Germany (author)
Amperometric Self-Referencing Ceramic Based Microelectrode Arrays for D-Serine Detection
- Article/chapterEnglish2018
Publisher, publication year, extent ...
- 2018-03-06
- MDPI,2018
- electronicrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-358576
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-358576URI
- https://doi.org/10.3390/bios8010020DOI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:142640444URI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- D-serine is the major D-amino acid in the mammalian central nervous system. As the dominant co-agonist of the endogenous synaptic NMDA receptor, D-serine plays a role in synaptic plasticity, learning, and memory. Alterations in D-serine are linked to neuropsychiatric disorders including schizophrenia. Thus, it is of increasing interest to monitor the concentration of D-serine in vivo as a relevant player in dynamic neuron-glia network activity. Here we present a procedure for amperometric detection of D-serine with self-referencing ceramic-based microelectrode arrays (MEAs) coated with D-amino acid oxidase from the yeast Rhodotorula gracilis (RgDAAO). We demonstrate in vitro D-serine recordings with a mean sensitivity of 8.61 +/- 0.83 pA/mu M to D-serine, a limit of detection (LOD) of 0.17 +/- 0.01 mu M, and a selectivity ratio of 80:1 or greater for D-serine over ascorbic acid (mean +/- SEM; n = 12) that can be used for freely moving studies.
Subject headings and genre
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- D-serine
- biosensor
- microelectrode array
- amperometry
- self-referencing
Added entries (persons, corporate bodies, meetings, titles ...)
- Konradsson-Geuken, ÅsaUppsala universitet,Institutionen för farmaceutisk biovetenskap,Karolinska Inst, Dept Physiol & Pharmacol, S-17177 Stockholm, Sweden(Swepub:uu)asako336 (author)
- Quintero, Jorge E.Univ Kentucky, CenMeT, Lexington, KY 40506 USA;Quanteon LLC, Nicholasville, KY 40356 USA (author)
- Marshall, LisaUniv Lubeck, Inst Expt & Clin Pharmacol & Toxicol, Ratzeburger Allee 160, D-23562 Lubeck, Germany (author)
- Univ Lubeck, Inst Expt & Clin Pharmacol & Toxicol, Ratzeburger Allee 160, D-23562 Lubeck, GermanyInstitutionen för farmaceutisk biovetenskap (creator_code:org_t)
Related titles
- In:Biosensors: MDPI8:12079-6374
Internet link
- https://doi.org/10.3390/bios8010020Fulltext
- https://uu.diva-portal.org/smash/get/diva2:1248637/FULLTEXT01.pdffulltext:print
- https://www.mdpi.com/2079-6374/8/1/20/pdf
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-358576
- https://doi.org/10.3390/bios8010020
- http://kipublications.ki.se/Default.aspx?queryparsed=id:142640444
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