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Mass Spectrometry R...
Mass Spectrometry Reveals the Direct Action of a Chemical Chaperone
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- Gault, Joseph (author)
- University of Oxford, United Kingdom
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Lianoudaki, Danai (author)
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- Kaldmäe, Margit (author)
- Karolinska Institutet
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- Kronqvist, Nina (author)
- Karolinska Institutet
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- Rising, Anna (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Karolinska Institutet,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB),Karolinska Institute
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- Johansson, Jan (author)
- Karolinska Institutet
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- Lohkamp, Bernhard (author)
- Karolinska Institutet
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- Laín, Sonia (author)
- Karolinska Institutet
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- Allison, Timothy M. (author)
- University of Canterbury, New Zealand
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Lane, David P. (author)
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- Marklund, Erik, Teknologie doktor, 1979- (author)
- Uppsala universitet,Biokemi,Erik Marklund
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- Landreh, Michael (author)
- Karolinska Institutet
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(creator_code:org_t)
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- 2018-07-05
- 2018
- English.
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In: The Journal of Physical Chemistry Letters. - : American Chemical Society (ACS). - 1948-7185. ; 9:14, s. 4082-4086
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Abstract
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- Despite their fundamental biological importance and therapeutic potential, the interactions between chemical chaperones and proteins remain difficult to capture due to their transient and nonspecific nature. Using a simple mass spectrometric assay, we are able to follow the interactions between proteins and the chemical chaperone trimethylamine-N-oxide (TMAO). In this manner, we directly observe that the counteraction of TMAO and the denaturant urea is driven by the exclusion of TMAO from the protein surface, whereas the surfactant lauryl dimethylamine-N-oxide cannot be displaced. Our results clearly demonstrate a direct chaperoning mechanism for TMAO, corroborating extensive computational studies, and pave the way for the use of nondenaturing mass spectrometry and related techniques to study chemical chaperones in molecular detail.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- Biokemi
- Biochemistry
- Kemi med inriktning mot biofysik
- Chemistry with specialization in Biophysics
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Gault, Joseph
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Lianoudaki, Dana ...
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Kaldmäe, Margit
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Kronqvist, Nina
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Rising, Anna
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Johansson, Jan
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show more...
-
Lohkamp, Bernhar ...
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Laín, Sonia
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Allison, Timothy ...
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Lane, David P.
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Marklund, Erik, ...
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Landreh, Michael
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
- Articles in the publication
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The Journal of P ...
- By the university
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Uppsala University
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Karolinska Institutet
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Swedish University of Agricultural Sciences