onr:"swepub:oai:DiVA.org:uu-369026"
Search: onr:"swepub:oai:DiVA.org:uu-369026" > Characterization of...
- 1 of 1
- Previous record
- Next record
- To hitlist
Characterization of epimerization and composition of heparin and dalteparin using a UHPLC-ESI-MS/MS method
-
- Zhang, Tianji (author)
- Beijing Univ Chem Technol, Beijing, Peoples R China;Natl Inst Metrol, Beijing, Peoples R China
-
- Liu, Xin (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
-
- Li, Hongmei (author)
- Natl Inst Metrol, Beijing, Peoples R China
- show more...
- show less...
- (creator_code:org_t)
- ELSEVIER SCI LTD, 2019
- 2019
- English.
- In: Carbohydrate Polymers. - : ELSEVIER SCI LTD. - 0144-8617 .- 1879-1344. ; 203, s. 87-94
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Heparin is a significant anticoagulant that has been used in clinic over decades. Although numerous efforts have been made to characterize the molecular structure of heparin and its derivatives for safety of the medicine, technical barriers still exist because of their structural complexity. In this study, we have established a method capable to evaluate both the epimerization and composition of heparin and dalteparin by a UHPLC-HILIC/WAXMS/MS approach. Ten major disaccharide building blocks retaining the epimerization configuration of parental heparin chains were generated and well separated, 9 of which were unambiguously identified. Isomer identifications were achieved through high-resolution tandem mass spectrometry analysis with reference to elaborately prepared standards. The method was successfully applied for the sameness study of generic dalteparins in combination with an isotopic labelling procedure. We believe this robust method maybe adapted to quality control in pharmaceutical production of heparin and LMWHs.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- Heparin
- Dalteparin
- Disaccharides
- UHPLC-HILIC/WAX-MS/MS
- Isotopic labelling
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Carbohydrate Polymers (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Zhang, Tianji
- Liu, Xin
- Li, Hongmei
- Wang, Zhangjie
- Chi, Lianli
- Li, Jin-Ping
- show more...
- Tan, Tianwei
- show less...
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Chemical Science ...
- and Analytical Chemi ...
- Articles in the publication
- Carbohydrate Pol ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
