onr:"swepub:oai:DiVA.org:uu-373508"
Search: onr:"swepub:oai:DiVA.org:uu-373508" > Kinetics of D-amino...
- 1 of 1
- Previous record
- Next record
- To hitlist
Kinetics of D-amino acid incorporation in translation
-
- Liljeruhm, Josefine (author)
- Uppsala universitet,Molekylärbiologi
-
- Wang, Jinfan, 1989- (author)
- Uppsala universitet,Molekylärbiologi
-
- Kwiatkowski, Marek (author)
- Uppsala universitet,Molekylärbiologi
- show more...
- show less...
- (creator_code:org_t)
- 2019-01-16
- 2019
- English.
- In: ACS Chemical Biology. - : American Chemical Society (ACS). - 1554-8929 .- 1554-8937. ; 14:2, s. 204-213
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Despite the stereospecificity of translation for l-amino acids (l-AAs) in vivo, synthetic biologists have enabled ribosomal incorporation of d-AAs in vitro toward encoding polypeptides with pharmacologically desirable properties. However, the steps in translation limiting d-AA incorporation need clarification. In this work, we compared d- and l-Phe incorporation in translation by quench-flow kinetics, measuring 250-fold slower incorporation into the dipeptide for the d isomer from a tRNAPhe-based adaptor (tRNAPheB). Incorporation was moderately hastened by tRNA body swaps and higher EF-Tu concentrations, indicating that binding by EF-Tu can be rate-limiting. However, from tRNAAlaB with a saturating concentration of EF-Tu, the slow d-Phe incorporation was unexpectedly very efficient in competition with incorporation of the l isomer, indicating fast binding to EF-Tu, fast binding of the resulting complex to the ribosome, and rate-limiting accommodation/peptide bond formation. Subsequent elongation with an l-AA was confirmed to be very slow and inefficient. This understanding helps rationalize incorporation efficiencies in vitro and stereospecific mechanisms in vivo and suggests approaches for improving incorporation.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- ACS Chemical Biology (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Liljeruhm, Josef ...
- Wang, Jinfan, 19 ...
- Kwiatkowski, Mar ...
- Sabari, Samudra
- Forster, Anthony ...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Medical Biotechn ...
- and Medical Biotechn ...
- Articles in the publication
- ACS Chemical Bio ...
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
