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Immunoglobulin Gene...
Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia : From Patient Material To Sequence Interpretation
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- Agathangelidis, Andreas (author)
- Karolinska Institutet,Ctr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece
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- Sutton, Lesley Ann (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden
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- Hadzidimitriou, Anastasia (author)
- Ctr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece
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- Tresoldi, Cristina (author)
- IRCCS San Raffaele Sci Inst, Div Immunol Transplantat & Infect, Milan, Italy
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- Langerak, Anton W. (author)
- Erasmus Univ, Med Ctr, Lab Med Immunol, Dept Immunol, Rotterdam, Netherlands
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- Belessi, Chrysoula (author)
- Nikea Gen Hosp, Hematol Dept, Piraeus, Greece
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- Davi, Frederic (author)
- Hop La Pitie Salpetriere, AP HP, Dept Hematol, Paris, France;UPMC Univ Paris 06, UMRS 1138, Paris, France
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- Rosenquist, Richard (author)
- Karolinska Institutet,Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden
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- Stamatopoulos, Kostas (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Ctr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece
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- Ghia, Paolo (author)
- IRCCS Ist Scientifico San Raffaele, Div Expt Oncol, Milan, Italy;Univ Vita Salute San Raffaele, Milan, Italy
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(creator_code:org_t)
- 2018-11-26
- 2018
- English.
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In: Journal of Visualized Experiments. - : JOURNAL OF VISUALIZED EXPERIMENTS. - 1940-087X. ; :141
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Abstract
Subject headings
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- During B cell maturation, the complex process of immunoglobulin (IG) gene V(D)J recombination coupled with somatic hypermutation (SHM) gives rise to a unique DNA sequence within each individual B cell. Since B cell malignancies result from the clonal expansion of a single cell, IG genes represent a unique molecular signature common to all the malignant cells within an individual patient; thus, IG gene rearrangements can be used as clonal markers. In addition to serving as an important clonal identifier, the IG gene sequence can act as a 'molecular timeline' since it is associated with specific developmental stages and hence reflects the history of the B cell involved in the neoplastic transformation. Moreover, for certain malignancies, in particular chronic lymphocytic leukemia (CLL), the IG gene sequence holds prognostic and potentially predictive capabilities. That said, extrapolating meaningful conclusions from IG gene sequence analysis would be impossible if robust methods and tools were not available to aid in their analysis. This article, drawing on the vast experience of the European Research Initiative on CLL (ERIC), details the technical aspects and essential requirements necessary to ensure reliable and reproducible IG gene sequence analysis in CLL, a test that is now recommended for all CLL patients prior to treatment. More specifically, the various analytical stages are described ranging from the identification of the clonotypic IG gene rearrangement and the determination of the nucleotide sequence to the accurate clinical interpretation of the IG gene sequence data.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Keyword
- Cancer Research
- Issue 141
- Chronic lymphocytic leukemia (CLL)
- somatic hypermutation (SHM)
- immunoglobulin
- immunoglobulin heavy variable (IGHV)
- complementarity determining region 3 (CDR3)
- International ImMunoGene Tics information system (IMGT)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Agathangelidis, ...
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Sutton, Lesley A ...
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Hadzidimitriou, ...
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Tresoldi, Cristi ...
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Langerak, Anton ...
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Belessi, Chrysou ...
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Davi, Frederic
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Rosenquist, Rich ...
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Stamatopoulos, K ...
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Ghia, Paolo
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Medical Genetics
- Articles in the publication
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Journal of Visua ...
- By the university
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Uppsala University
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Karolinska Institutet