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Immunoglobulin Gene...
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Agathangelidis, AndreasKarolinska Institutet,Ctr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece
(author)
Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia : From Patient Material To Sequence Interpretation
- Article/chapterEnglish2018
Publisher, publication year, extent ...
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2018-11-26
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JOURNAL OF VISUALIZED EXPERIMENTS,2018
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electronicrdacarrier
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LIBRIS-ID:oai:DiVA.org:uu-376902
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-376902URI
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https://doi.org/10.3791/57787DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:140119258URI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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De två första författarna delar förstaförfattarskapet.
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During B cell maturation, the complex process of immunoglobulin (IG) gene V(D)J recombination coupled with somatic hypermutation (SHM) gives rise to a unique DNA sequence within each individual B cell. Since B cell malignancies result from the clonal expansion of a single cell, IG genes represent a unique molecular signature common to all the malignant cells within an individual patient; thus, IG gene rearrangements can be used as clonal markers. In addition to serving as an important clonal identifier, the IG gene sequence can act as a 'molecular timeline' since it is associated with specific developmental stages and hence reflects the history of the B cell involved in the neoplastic transformation. Moreover, for certain malignancies, in particular chronic lymphocytic leukemia (CLL), the IG gene sequence holds prognostic and potentially predictive capabilities. That said, extrapolating meaningful conclusions from IG gene sequence analysis would be impossible if robust methods and tools were not available to aid in their analysis. This article, drawing on the vast experience of the European Research Initiative on CLL (ERIC), details the technical aspects and essential requirements necessary to ensure reliable and reproducible IG gene sequence analysis in CLL, a test that is now recommended for all CLL patients prior to treatment. More specifically, the various analytical stages are described ranging from the identification of the clonotypic IG gene rearrangement and the determination of the nucleotide sequence to the accurate clinical interpretation of the IG gene sequence data.
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Sutton, Lesley AnnUppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden(Swepub:uu)lessu455
(author)
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Hadzidimitriou, AnastasiaCtr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece
(author)
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Tresoldi, CristinaIRCCS San Raffaele Sci Inst, Div Immunol Transplantat & Infect, Milan, Italy
(author)
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Langerak, Anton W.Erasmus Univ, Med Ctr, Lab Med Immunol, Dept Immunol, Rotterdam, Netherlands
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Belessi, ChrysoulaNikea Gen Hosp, Hematol Dept, Piraeus, Greece
(author)
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Davi, FredericHop La Pitie Salpetriere, AP HP, Dept Hematol, Paris, France;UPMC Univ Paris 06, UMRS 1138, Paris, France
(author)
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Rosenquist, RichardKarolinska Institutet,Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden(Swepub:uu)richrose
(author)
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Stamatopoulos, KostasUppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Ctr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece(Swepub:uu)kosst292
(author)
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Ghia, PaoloIRCCS Ist Scientifico San Raffaele, Div Expt Oncol, Milan, Italy;Univ Vita Salute San Raffaele, Milan, Italy
(author)
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Karolinska InstitutetCtr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece
(creator_code:org_t)
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In:Journal of Visualized Experiments: JOURNAL OF VISUALIZED EXPERIMENTS:1411940-087X
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