Search: onr:"swepub:oai:DiVA.org:uu-376902" > Immunoglobulin Gene...
Fältnamn | Indikatorer | Metadata |
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000 | 05422naa a2200577 4500 | |
001 | oai:DiVA.org:uu-376902 | |
003 | SwePub | |
008 | 190212s2018 | |||||||||||000 ||eng| | |
009 | oai:prod.swepub.kib.ki.se:140119258 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3769022 URI |
024 | 7 | a https://doi.org/10.3791/577872 DOI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1401192582 URI |
040 | a (SwePub)uud (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Agathangelidis, Andreasu Karolinska Institutet,Ctr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece4 aut |
245 | 1 0 | a Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia :b From Patient Material To Sequence Interpretation |
264 | c 2018-11-26 | |
264 | 1 | b JOURNAL OF VISUALIZED EXPERIMENTS,c 2018 |
338 | a electronic2 rdacarrier | |
500 | a De två första författarna delar förstaförfattarskapet. | |
520 | a During B cell maturation, the complex process of immunoglobulin (IG) gene V(D)J recombination coupled with somatic hypermutation (SHM) gives rise to a unique DNA sequence within each individual B cell. Since B cell malignancies result from the clonal expansion of a single cell, IG genes represent a unique molecular signature common to all the malignant cells within an individual patient; thus, IG gene rearrangements can be used as clonal markers. In addition to serving as an important clonal identifier, the IG gene sequence can act as a 'molecular timeline' since it is associated with specific developmental stages and hence reflects the history of the B cell involved in the neoplastic transformation. Moreover, for certain malignancies, in particular chronic lymphocytic leukemia (CLL), the IG gene sequence holds prognostic and potentially predictive capabilities. That said, extrapolating meaningful conclusions from IG gene sequence analysis would be impossible if robust methods and tools were not available to aid in their analysis. This article, drawing on the vast experience of the European Research Initiative on CLL (ERIC), details the technical aspects and essential requirements necessary to ensure reliable and reproducible IG gene sequence analysis in CLL, a test that is now recommended for all CLL patients prior to treatment. More specifically, the various analytical stages are described ranging from the identification of the clonotypic IG gene rearrangement and the determination of the nucleotide sequence to the accurate clinical interpretation of the IG gene sequence data. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Medicinsk genetik0 (SwePub)301072 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Medical Genetics0 (SwePub)301072 hsv//eng |
653 | a Cancer Research | |
653 | a Issue 141 | |
653 | a Chronic lymphocytic leukemia (CLL) | |
653 | a somatic hypermutation (SHM) | |
653 | a immunoglobulin | |
653 | a immunoglobulin heavy variable (IGHV) | |
653 | a complementarity determining region 3 (CDR3) | |
653 | a International ImMunoGene Tics information system (IMGT) | |
700 | 1 | a Sutton, Lesley Annu Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden4 aut0 (Swepub:uu)lessu455 |
700 | 1 | a Hadzidimitriou, Anastasiau Ctr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece4 aut |
700 | 1 | a Tresoldi, Cristinau IRCCS San Raffaele Sci Inst, Div Immunol Transplantat & Infect, Milan, Italy4 aut |
700 | 1 | a Langerak, Anton W.u Erasmus Univ, Med Ctr, Lab Med Immunol, Dept Immunol, Rotterdam, Netherlands4 aut |
700 | 1 | a Belessi, Chrysoulau Nikea Gen Hosp, Hematol Dept, Piraeus, Greece4 aut |
700 | 1 | a Davi, Fredericu Hop La Pitie Salpetriere, AP HP, Dept Hematol, Paris, France;UPMC Univ Paris 06, UMRS 1138, Paris, France4 aut |
700 | 1 | a Rosenquist, Richardu Karolinska Institutet,Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden4 aut0 (Swepub:uu)richrose |
700 | 1 | a Stamatopoulos, Kostasu Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för immunologi, genetik och patologi,Ctr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece4 aut0 (Swepub:uu)kosst292 |
700 | 1 | a Ghia, Paolou IRCCS Ist Scientifico San Raffaele, Div Expt Oncol, Milan, Italy;Univ Vita Salute San Raffaele, Milan, Italy4 aut |
710 | 2 | a Karolinska Institutetb Ctr Res & Technol Hellas, Inst Appl Biosci, Thessaloniki, Greece4 org |
773 | 0 | t Journal of Visualized Experimentsd : JOURNAL OF VISUALIZED EXPERIMENTSg :141q :141x 1940-087X |
856 | 4 | u https://doi.org/10.3791/57787y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1287797/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 | u https://www.jove.com/pdf/57787/immunoglobulin-gene-sequence-analysis-chronic-lymphocytic-leukemia |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-376902 |
856 | 4 8 | u https://doi.org/10.3791/57787 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:140119258 |
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