Search: onr:"swepub:oai:DiVA.org:uu-379240" >
Comparative Analysi...
Comparative Analysis of Dry and Wet Porometry Methods for Characterization of Regular and Cross-Linked Virus Removal Filter Papers
-
- Gustafsson, Simon, 1988- (author)
- Uppsala universitet,Nanoteknologi och funktionella material
-
- Westermann, Frank (author)
- Charles River Biopharmaceut Serv, Gottfried Hagen Str 20, D-51105 Cologne, Germany
-
- Hanrieder, Tobias (author)
- Charles River Biopharmaceut Serv, Gottfried Hagen Str 20, D-51105 Cologne, Germany
-
show more...
-
- Jung, Laura (author)
- Charles River Biopharmaceut Serv, Gottfried Hagen Str 20, D-51105 Cologne, Germany
-
- Ruppach, Horst (author)
- Charles River Biopharmaceut Serv, Gottfried Hagen Str 20, D-51105 Cologne, Germany
-
- Mihranyan, Albert, 1978- (author)
- Uppsala universitet,Nanoteknologi och funktionella material
-
show less...
-
(creator_code:org_t)
- 2018-12-20
- 2019
- English.
-
In: Membranes. - : MDPI AG. - 2077-0375. ; 9:1
- Related links:
-
https://uu.diva-port... (primary) (Raw object)
-
show more...
-
https://res.mdpi.com...
-
https://urn.kb.se/re...
-
https://doi.org/10.3...
-
show less...
Abstract
Subject headings
Close
- Pore-size distribution (PSD) is the most critical parameter for size-exclusion virus removal filters. Yet, different dry- and wet-state porometry methods yield different pore-size values. The goal of this work is to conduct comparative analysis of nitrogen gas sorption (NGSP), liquid-liquid and cryoporometry with differential scanning calorimetry (CP-DSC) methods with respect to characterization of regular and cross-linked virus removal filter paper based on cellulose nanofibers, i.e. the mille-feuille filter. The filters were further characterized with atomic force and scanning electron microscopy. Finally, the removal of the worst-case model virus, i.e. minute virus of mice (MVM; 20 nm, nonenveloped parvovirus) was evaluated. The results revealed that there is no difference of the obtained PSDs between the wet methods, i.e. DSC and liquid-liquid porometry (LLP), as well as no difference between the regular and cross-linked filters regardless of method. MVM filtration at different trans membrane pressure (TMP) revealed strong dependence of the virus removal capability on applied pressure. It was further observed that cross-linking filters showed enhanced virus removal, especially at lower TMP. In all, the results of this study highlight the complex nature of virus capture in size-exclusion filters.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Nanoteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Nano-technology (hsv//eng)
Keyword
- gas sorption porometry
- liquid-liquid porometry
- cryoporometry
- mille-feuille filter
- parvovirus
- virus removal filter
- Engineering Science with specialization in Nanotechnology and Functional Materials
- Teknisk fysik med inriktning mot nanoteknologi och funktionella material
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
Membranes
(Search for host publication in LIBRIS)
To the university's database