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Dual roles of hepar...
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Aldi, SilviaKarolinska Institutet
(author)
Dual roles of heparanase in human carotid plaque calcification
- Article/chapterEnglish2019
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ELSEVIER IRELAND LTD,2019
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LIBRIS-ID:oai:DiVA.org:uu-381114
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-381114URI
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https://doi.org/10.1016/j.atherosclerosis.2018.12.027DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:140512227URI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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Background and aims: Calcification is a hallmark of advanced atherosclerosis and an active process akin to bone remodeling. Heparanase (HPSE) is an endo-beta-glucuronidase, which cleaves glycosaminoglycan chains of heparan sulfate proteoglycans. The role of HPSE is controversial in osteogenesis and bone remodeling while it is unexplored in vascular calcification. Previously, we reported upregulation of HPSE in human carotid endarterectomies from symptomatic patients and showed correlation of HPSE expression with markers of inflammation and increased thrombogenicity. The present aim is to investigate HPSE expression in relation to genes associated with osteogenesis and osteolysis and the effect of elevated HPSE expression on calcification and osteolysis in vitro.Methods: Transcriptomic and immunohistochemical analyses were performed using the Biobank of Karolinska Endarterectomies (BiKE). In vitro calcification and osteolysis were analysed in human carotid smooth muscle cells overexpressing HPSE and bone marrow-derived osteoclasts from HPSE-transgenic mice respectively.Results: HPSE expression correlated primarily with genes coupled to osteoclast differentiation and function in human carotid atheromas. HPSE was expressed in osteoclast-like cells in atherosclerotic lesions, and HPSE-transgenic bone marrow-derived osteoclasts displayed a higher osteolytic activity compared to wild-type cells. Contrarily, human carotid SMCs with an elevated HPSE expression demonstrated markedly increased mineralization upon osteogenic differentiation.Conclusions: We suggest that HPSE may have dual functions in vascular calcification, depending on the stage of the disease and presence of inflammatory cells. While HPSE plausibly enhances mineralization and osteogenic differentiation of vascular smooth muscle cells, it is associated with inflammation-induced osteoclast differentiation and activity in advanced atherosclerotic plaques.
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Eriksson, LinneaKarolinska Inst, Dept Mol Med & Surg, Bioclinicum J8 20, S-17164 Solna, Sweden
(author)
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Kronqvist, MalinKarolinska Institutet
(author)
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Lengquist, MarietteKarolinska Institutet
(author)
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Löfling, MarieKarolinska Institutet
(author)
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Folkersen, LasseTech Univ Denmark, Ctr Biol Sequence Anal, Copenhagen, Denmark
(author)
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Matic, Ljubica P.Karolinska Inst, Dept Mol Med & Surg, Bioclinicum J8 20, S-17164 Solna, Sweden
(author)
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Maegdefessel, LarsKarolinska Institutet
(author)
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Grinnemo, Karl-HenrikKarolinska Inst, Dept Mol Med & Surg, Bioclinicum J8 20, S-17164 Solna, Sweden
(author)
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Li, Jin-PingUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)jinpili
(author)
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Österholm, C.Karolinska Institutet
(author)
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Hedin, UlfKarolinska Institutet
(author)
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Karolinska InstitutetKarolinska Inst, Dept Mol Med & Surg, Bioclinicum J8 20, S-17164 Solna, Sweden
(creator_code:org_t)
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In:Atherosclerosis: ELSEVIER IRELAND LTD283, s. 127-1360021-91501879-1484
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Aldi, Silvia
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Eriksson, Linnea
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Kronqvist, Malin
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Lengquist, Marie ...
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Löfling, Marie
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Folkersen, Lasse
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Matic, Ljubica P ...
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Maegdefessel, La ...
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Grinnemo, Karl-H ...
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Li, Jin-Ping
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Österholm, C.
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Hedin, Ulf
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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Atherosclerosis
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Uppsala University
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Karolinska Institutet