onr:"swepub:oai:DiVA.org:uu-390195"
Search: onr:"swepub:oai:DiVA.org:uu-390195" > Divergent Effects o...
- 1 of 1
- Previous record
- Next record
- To hitlist
Divergent Effects of Acute and Prolonged Interleukin 33 Exposure on Mast Cell IgE-Mediated Functions
-
- Rönnberg, Elin (author)
- Karolinska Institutet,Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Immunol & Allergy Unit, Solna, Sweden
-
- Ghaib, Avan (author)
- Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Immunol & Allergy Unit, Solna, Sweden;Univ Sulaimani, Coll Med, Dept Microbiol, Sulaimani, Iraq
-
- Ceriol, Carlos (author)
- Karolinska Univ Hosp, Karolinska Inst, Dept Med Solna, Immunol & Allergy Unit, Solna, Sweden
- show more...
- Orre, Ann-Charlotte (author)
- show less...
- (creator_code:org_t)
- 2019-06-19
- 2019
- English.
- In: Frontiers in Immunology. - : FRONTIERS MEDIA SA. - 1664-3224. ; 10
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Background: Epithelial cytokines, including IL-33 and Thymic stromal lymphopoietin (TSLP), have attracted interest because of their roles in chronic allergic inflammation-related conditions such as asthma. Mast cells are one of the major targets of IL-33, to which they respond by secreting cytokines. Most studies performed thus far have investigated the acute effects of IL-33 on mast cells. In the current study, we investigated how acute vs. prolonged exposure of mast cells to IL-33 and TSLP affects mediator synthesis and IgE-mediated activation.Methods: Human lung mast cells (HLMCs), cord blood-derived mast cells (CBMCs), and the ROSA mast cell line were used for this study. Receptor expression and the levels of mediators were measured after treatment with IL-33 and/or TSLP.Results: IL-33 induced the release of cytokines. Prolonged exposure to IL-33 increased while TSLP reduced intracellular levels of tryptase. Acute IL-33 treatment strongly potentiated IgE-mediated activation. In contrast, 4 days of exposure to IL-33 decreased IgE-mediated activation, an effect that was accompanied by a reduction in Fc epsilon RI expression.Conclusion: We show that IL-33 plays dual roles in mast cells, in which its acute effects include cytokine release and the potentiation of IgE-mediated degranulation, whereas prolonged exposure to IL-33 reduces IgE-mediated activation. We conclude that mast cells act quickly in response to the alarmin IL-33 to initiate an acute inflammatory response, whereas extended exposure to IL-33 during prolonged inflammation reduces IgE-mediated responses. This negative feedback effect suggests the presence of a novel regulatory pathway that modulates IgE-mediated human mast cell responses.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Fc epsilon RI
- IgE
- IL-33
- mast cells
- TSLP
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Frontiers in Immunology (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Rönnberg, Elin
- Ghaib, Avan
- Ceriol, Carlos
- Enoksson, Mattia ...
- Arock, Michel
- Säfholm, Jesper
- show more...
- Orre, Ann-Charlo ...
- Al-Ameri, Mamdoh
- Adner, Mikael
- Dahlen, Sven-Eri ...
- Ekoff, Maria
- Nilsson, Gunnar
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Immunology in th ...
- Articles in the publication
- Frontiers in Imm ...
- By the university
- Uppsala University
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
