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Giardia intestinali...
Giardia intestinalis cystatin is a potent inhibitor of papain, parasite cysteine proteases and, to a lesser extent, human cathepsin B
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- Liu, Jingyi (author)
- Uppsala universitet,Mikrobiologi
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- Svärd, Staffan (author)
- Uppsala universitet,Mikrobiologi
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- Klotz, Christian (author)
- Robert Koch Inst, Dept Mycot & Parasit Agents & Mycobacteria FG16, Seestr 10, D-13353 Berlin, Germany
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(creator_code:org_t)
- 2019-05-22
- 2019
- English.
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In: FEBS Letters. - : John Wiley & Sons. - 0014-5793 .- 1873-3468. ; 593:12, s. 1313-1325
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https://febs.onlinel...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Cystatins are important regulators of papain-like cysteine proteases. In the protozoan parasite Giardia intestinalis, papain-like cysteine proteases play an essential role in the parasite's biology and pathogenicity. Here, we characterized a cysteine protease inhibitor of G. intestinalis that belongs to type-I-cystatins. The parasite cystatin is shown to be a strong inhibitor of papain (K-i approximate to 0.3 nm) and three parasite cysteine proteases (CP14019, CP16160 and CP16779, K-i approximate to 0.9-5.8 nm), but a weaker inhibitor of human cathepsin B (K-i approximate to 79.9 nm). The protein localizes mainly in the cytoplasm. Together, these data suggest that cystatin of G. intestinalis plays a role in the regulation of cysteine protease activities in the parasite and, possibly, in the interaction with the host.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- cystatin
- cysteine protease inhibitor
- cysteine proteases
- Giardia intestinalis
- protozoa
Publication and Content Type
- ref (subject category)
- art (subject category)
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