Thromboinflammatory changes in plasma proteome of pregnant women with PCOS detected by quantitative label-free proteomics

Arffman, R. K.Univ Oulu, Oulu Univ Hosp, Med Res Ctr, Dept Obstet & Gynecol,PEDEGO Res Unit, Oulu, Finland (author)

Polycystic ovary syndrome (PCOS) is the most common endocrinological disorder of fertile-aged women. Several adverse pregnancy outcomes and abnormalities of the placenta have been associated with PCOS. By using quantitative label-free proteomics we investigated whether changes in the plasma proteome of pregnant women with PCOS could elucidate the mechanisms behind the pathologies observed in PCOS pregnancies. A total of 169 proteins with >= 2 unique peptides were detected to be differentially expressed between women with PCOS (n = 7) and matched controls (n = 20) at term of pregnancy, out of which 35 were significant (p-value < 0.05). A pathway analysis revealed that networks related to humoral immune responses, inflammatory responses, cardiovascular disease and cellular growth and proliferation were affected by PCOS. Classification of cases and controls was carried out using principal component analysis, orthogonal projections on latent structure-discriminant analysis (OPLS-DA), hierarchical clustering, self-organising maps and ROC-curve analysis. The most significantly enriched proteins in PCOS were properdin and insulin-like growth factor II. In the dataset, properdin had the best predictive accuracy for PCOS (AUC=1). Additionally, properdin abundances correlated with AMH levels in pregnant women.

Saraswat, M.Univ Helsinki, Haartman Inst, Transplantat Lab, Helsinki, Finland;Helsinki Univ Hosp, HUSLAB, Helsinki, Finland (author)
Joenväärä, S.Univ Helsinki, Haartman Inst, Transplantat Lab, Helsinki, Finland;Helsinki Univ Hosp, HUSLAB, Helsinki, Finland (author)
Khatun, M.Univ Oulu, Oulu Univ Hosp, Med Res Ctr, Dept Obstet & Gynecol,PEDEGO Res Unit, Oulu, Finland (author)
Agarwal, R.All India Inst Med Sci, Dept Reprod Biol, New Delhi 110029, India (author)
Tohmola, T.Univ Helsinki, Haartman Inst, Transplantat Lab, Helsinki, Finland;Helsinki Univ Hosp, HUSLAB, Helsinki, Finland (author)
Sundström Poromaa, Inger,1964-Uppsala universitet,Reproduktiv hälsa(Swepub:uu)inspo702 (author)
Renkonen, R.Univ Helsinki, Haartman Inst, Transplantat Lab, Helsinki, Finland;Helsinki Univ Hosp, HUSLAB, Helsinki, Finland (author)
Piltonen, T. T.Univ Oulu, Oulu Univ Hosp, Med Res Ctr, Dept Obstet & Gynecol,PEDEGO Res Unit, Oulu, Finland (author)
Univ Oulu, Oulu Univ Hosp, Med Res Ctr, Dept Obstet & Gynecol,PEDEGO Res Unit, Oulu, FinlandUniv Helsinki, Haartman Inst, Transplantat Lab, Helsinki, Finland;Helsinki Univ Hosp, HUSLAB, Helsinki, Finland (creator_code:org_t)

By the author/editor: Arffman, R. K.; Saraswat, M.; Joenväärä, S.; Khatun, M.; Agarwal, R.; Tohmola, T.; show more...; Sundström Poroma ...; Renkonen, R.; Piltonen, T. T.; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Obstetrics Gynae ...

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