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Impact of Q-Griffit...
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Günaydin, GökceKarolinska Institutet
(author)
Impact of Q-Griffithsin anti-HIV microbicide gel in non-human primates : In situ analyses of epithelial and immune cell markers in rectal mucosa
- Article/chapterEnglish2019
Publisher, publication year, extent ...
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2019-12-02
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NATURE PUBLISHING GROUP,2019
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electronicrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-400679
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-400679URI
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https://doi.org/10.1038/s41598-019-54493-4DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:142434748URI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Natural-product derived lectins can function as potent viral inhibitors with minimal toxicity as shown in vitro and in small animal models. We here assessed the effect of rectal application of an anti-HIV lectin-based microbicide Q-Griffithsin (Q-GRFT) in rectal tissue samples from rhesus macaques. E-cadherin(+) cells, CD4(+) cells and total mucosal cells were assessed using in situ staining combined with a novel customized digital image analysis platform. Variations in cell numbers between baseline, placebo and Q-GRFT treated samples were analyzed using random intercept linear mixed effect models. The frequencies of rectal E-cadherin(+) cells remained stable despite multiple tissue samplings and Q-GRFT gel (0.1%, 0.3% and 1%, respectively) treatment. Whereas single dose application of Q-GRFT did not affect the frequencies of rectal CD4(+) cells, multi-dose Q-GRFT caused a small, but significant increase of the frequencies of intra-epithelial CD4(+) cells (placebo: median 4%; 1% Q-GRFT: median 7%) and of the CD4(+) lamina propria cells (placebo: median 30%; 0.1-1% Q-GRFT: median 36-39%). The resting time between sampling points were further associated with minor changes in the total and CD4(+) rectal mucosal cell levels. The results add to general knowledge of in vivo evaluation of anti-HIV microbicide application concerning cellular effects in rectal mucosa.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Edfeldt, GabriellaKarolinska Univ Hosp, Karolinska Inst, Ctr Mol Med, Dept Med Solna,Div Infect Dis, Stockholm, Sweden
(author)
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Garber, David A.CDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA
(author)
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Asghar, MuhammadKarolinska Institutet
(author)
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Noel-Romas, LauraPubl Hlth Agcy Canada, JC Wilt Infect Dis Res Ctr, Natl HIV & Retrovirol Labs, Winnipeg, MB, Canada;Univ Manitoba, Dept Obstet & Gynecol, Winnipeg, MB, Canada;Univ Manitoba, Dept Med Microbiol, Winnipeg, MB, Canada
(author)
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Burgener, AdamKarolinska Institutet
(author)
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Wählby, Carolina,professor,1974-Uppsala universitet,Avdelningen för visuell information och interaktion,Science for Life Laboratory, SciLifeLab,Bildanalys och människa-datorinteraktion(Swepub:uu)cli05194
(author)
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Wang, LinMagee Womens Res Inst, Pittsburgh, PA USA
(author)
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Rohan, Lisa C.Magee Womens Res Inst, Pittsburgh, PA USA;Univ Pittsburgh, Sch Pharm, Pittsburgh, PA USA
(author)
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Guenthner, PatriciaCDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA
(author)
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Mitchell, JamesCDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA
(author)
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Matoba, NobuyukiUniv Louisville, Ctr Predict Med, Louisville, KY 40292 USA;Univ Louisville, Dept Pharmacol & Toxicol, Louisville, KY 40292 USA;Univ Louisville, James Graham Brown Canc Ctr, Louisville, KY 40292 USA
(author)
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McNicholl, Janet M.CDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA
(author)
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Palmer, Kenneth E.Univ Louisville, Ctr Predict Med, Louisville, KY 40292 USA;Univ Louisville, Dept Pharmacol & Toxicol, Louisville, KY 40292 USA;Univ Louisville, James Graham Brown Canc Ctr, Louisville, KY 40292 USA
(author)
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Tjernlund, AnnelieKarolinska Institutet
(author)
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Broliden, KristinaKarolinska Institutet
(author)
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Karolinska InstitutetKarolinska Univ Hosp, Karolinska Inst, Ctr Mol Med, Dept Med Solna,Div Infect Dis, Stockholm, Sweden
(creator_code:org_t)
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In:Scientific Reports: NATURE PUBLISHING GROUP92045-2322
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