SwePub
Sök i LIBRIS databas

  Utökad sökning

onr:"swepub:oai:DiVA.org:uu-400679"
 

Sökning: onr:"swepub:oai:DiVA.org:uu-400679" > Impact of Q-Griffit...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00005434naa a2200541 4500
001oai:DiVA.org:uu-400679
003SwePub
008200102s2019 | |||||||||||000 ||eng|
009oai:prod.swepub.kib.ki.se:142434748
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4006792 URI
0247 a https://doi.org/10.1038/s41598-019-54493-42 DOI
0247 a http://kipublications.ki.se/Default.aspx?queryparsed=id:1424347482 URI
040 a (SwePub)uud (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Günaydin, Gökceu Karolinska Institutet4 aut
2451 0a Impact of Q-Griffithsin anti-HIV microbicide gel in non-human primates :b In situ analyses of epithelial and immune cell markers in rectal mucosa
264 c 2019-12-02
264 1b NATURE PUBLISHING GROUP,c 2019
338 a electronic2 rdacarrier
520 a Natural-product derived lectins can function as potent viral inhibitors with minimal toxicity as shown in vitro and in small animal models. We here assessed the effect of rectal application of an anti-HIV lectin-based microbicide Q-Griffithsin (Q-GRFT) in rectal tissue samples from rhesus macaques. E-cadherin(+) cells, CD4(+) cells and total mucosal cells were assessed using in situ staining combined with a novel customized digital image analysis platform. Variations in cell numbers between baseline, placebo and Q-GRFT treated samples were analyzed using random intercept linear mixed effect models. The frequencies of rectal E-cadherin(+) cells remained stable despite multiple tissue samplings and Q-GRFT gel (0.1%, 0.3% and 1%, respectively) treatment. Whereas single dose application of Q-GRFT did not affect the frequencies of rectal CD4(+) cells, multi-dose Q-GRFT caused a small, but significant increase of the frequencies of intra-epithelial CD4(+) cells (placebo: median 4%; 1% Q-GRFT: median 7%) and of the CD4(+) lamina propria cells (placebo: median 30%; 0.1-1% Q-GRFT: median 36-39%). The resting time between sampling points were further associated with minor changes in the total and CD4(+) rectal mucosal cell levels. The results add to general knowledge of in vivo evaluation of anti-HIV microbicide application concerning cellular effects in rectal mucosa.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
7001 a Edfeldt, Gabriellau Karolinska Univ Hosp, Karolinska Inst, Ctr Mol Med, Dept Med Solna,Div Infect Dis, Stockholm, Sweden4 aut
7001 a Garber, David A.u CDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA4 aut
7001 a Asghar, Muhammadu Karolinska Institutet4 aut
7001 a Noel-Romas, Laurau Publ Hlth Agcy Canada, JC Wilt Infect Dis Res Ctr, Natl HIV & Retrovirol Labs, Winnipeg, MB, Canada;Univ Manitoba, Dept Obstet & Gynecol, Winnipeg, MB, Canada;Univ Manitoba, Dept Med Microbiol, Winnipeg, MB, Canada4 aut
7001 a Burgener, Adamu Karolinska Institutet4 aut
7001 a Wählby, Carolina,c professor,d 1974-u Uppsala universitet,Avdelningen för visuell information och interaktion,Science for Life Laboratory, SciLifeLab,Bildanalys och människa-datorinteraktion4 aut0 (Swepub:uu)cli05194
7001 a Wang, Linu Magee Womens Res Inst, Pittsburgh, PA USA4 aut
7001 a Rohan, Lisa C.u Magee Womens Res Inst, Pittsburgh, PA USA;Univ Pittsburgh, Sch Pharm, Pittsburgh, PA USA4 aut
7001 a Guenthner, Patriciau CDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA4 aut
7001 a Mitchell, Jamesu CDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA4 aut
7001 a Matoba, Nobuyukiu Univ Louisville, Ctr Predict Med, Louisville, KY 40292 USA;Univ Louisville, Dept Pharmacol & Toxicol, Louisville, KY 40292 USA;Univ Louisville, James Graham Brown Canc Ctr, Louisville, KY 40292 USA4 aut
7001 a McNicholl, Janet M.u CDC, Natl Ctr HIV AIDS Viral Hepatitis Sexually Transm, Div HIV AIDS Prevent, Lab Branch, Atlanta, GA 30333 USA4 aut
7001 a Palmer, Kenneth E.u Univ Louisville, Ctr Predict Med, Louisville, KY 40292 USA;Univ Louisville, Dept Pharmacol & Toxicol, Louisville, KY 40292 USA;Univ Louisville, James Graham Brown Canc Ctr, Louisville, KY 40292 USA4 aut
7001 a Tjernlund, Annelieu Karolinska Institutet4 aut
7001 a Broliden, Kristinau Karolinska Institutet4 aut
7102 a Karolinska Institutetb Karolinska Univ Hosp, Karolinska Inst, Ctr Mol Med, Dept Med Solna,Div Infect Dis, Stockholm, Sweden4 org
7730 t Scientific Reportsd : NATURE PUBLISHING GROUPg 9q 9x 2045-2322
8564 u https://doi.org/10.1038/s41598-019-54493-4y Fulltext
8564 u https://uu.diva-portal.org/smash/get/diva2:1382181/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 u https://www.nature.com/articles/s41598-019-54493-4.pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-400679
8564 8u https://doi.org/10.1038/s41598-019-54493-4
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:142434748

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy