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Immunomodulatory ef...
Immunomodulatory effects of interferon-gamma on human fetal cardiac mesenchymal stromal cells
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- Grinnemo, Karl-Henrik (author)
- Uppsala universitet,Thoraxkirurgi,Karolinska Inst, BioClinicum J10 20, Dept Mol Med & Surg, SE-17164 Solna, Sweden
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- Löfling, Marie (author)
- Karolinska Inst, BioClinicum J10 20, Dept Mol Med & Surg, SE-17164 Solna, Sweden
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- Nathanson, Lubov (author)
- Nova Southeastern Univ, Dr Kiran C Patel Coll Osteopath Med, Inst Neuroimmune Med, Davie, FL USA
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- Baumgartner, Roland (author)
- Karolinska Inst, Ctr Mol Med, Dept Med Solna, Cardiovasc Med Unit, SE-17164 Solna, Sweden
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- Ketelhuth, Daniel F. J. (author)
- Karolinska Inst, Ctr Mol Med, Dept Med Solna, Cardiovasc Med Unit, SE-17164 Solna, Sweden
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- Beljanski, Vladimir (author)
- Nova Southeastern Univ, Cell Therapy Inst, Dr Kiran C Patel Coll Allopath Med, Davie, FL USA
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- Davies, Lindsay C. (author)
- Karolinska Inst, Dept Lab Med, SE-14152 Huddinge, Sweden
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- Österholm, Cecilia (author)
- Karolinska Inst, BioClinicum J10 20, Dept Mol Med & Surg, SE-17164 Solna, Sweden
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(creator_code:org_t)
- 2019-12-04
- 2019
- English.
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In: Stem Cell Research & Therapy. - : BMC. - 1757-6512. ; 10
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Abstract
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- Background: Mesenchymal stromal cells (MSCs), due to their regenerative and immunomodulatory properties, are therapeutically used for diseases, including heart failure. As early gestational-phase embryonic tissues exhibit extraordinary regenerative potential, fetal MSCs exposed to inflammation offer a unique opportunity to evaluate molecular mechanisms underlying preferential healing, and investigate their inherent abilities to communicate with the immune system during development. The principal aim of this study was to evaluate the effects of interferon-gamma (IFN gamma) on the immunomodulatory effects of first-trimester human fetal cardiac (hfc)-MSCs.Methods: hfcMSCs (gestational week 8) were exposed to IFN gamma, with subsequent analysis of the whole transcriptome, based on RNA sequencing. Exploration of surface-expressed immunoregulatory mediators and modulation of T cell responses were performed by flow cytometry. Presence and activity of soluble mediators were assessed by ELISA or high-performance liquid chromatography.Results: Stimulation of hfcMSCs with IFN gamma revealed significant transcriptional changes, particularly in respect to the expression of genes belonging to antigen presentation pathways, cell cycle control, and interferon signaling. Expression of immunomodulatory genes and associated functional changes, including indoleamine 2,3-dioxygenase activity, and regulation of T cell activation and proliferation via programmed cell death protein (PD)-1 and its ligands PD-L1 and PD-L2, were significantly upregulated. These immunoregulatory molecules diminished rapidly upon withdrawal of inflammatory stimulus, indicating a high degree of plasticity by hfcMSCs.Conclusions: To our knowledge, this is the first study performing a systematic evaluation of inflammatory responses and immunoregulatory properties of first-trimester cardiac tissue. In summary, our study demonstrates the dynamic responsiveness of hfcMSCs to inflammatory stimuli. Further understanding as to the immunoregulatory properties of hfcMSCs may be of benefit in the development of novel stromal cell therapeutics for cardiovascular disease.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Mesenchymal stromal cells
- Fetal hearts
- ISL1
- Inflammation
- Immunomodulation
- Programmed cell death protein 1
Publication and Content Type
- ref (subject category)
- art (subject category)
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