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Recent advances in ...
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Jex, Aaron R.Walter & Eliza Hall Inst Med Res, Populat Hlth & Immun Div, Melbourne, Vic, Australia.;Univ Melbourne, Fac Vet & Agr Sci, Parkville, Vic, Australia.
(författare)
Recent advances in functional research in Giardia intestinalis
- Artikel/kapitelEngelska2020
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Elsevier,2020
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LIBRIS-ID:oai:DiVA.org:uu-412347
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-412347URI
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https://doi.org/10.1016/bs.apar.2019.12.002DOI
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Språk:engelska
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Sammanfattning på:engelska
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Aaron R. Jex, Staffan Svärd and Scott C. Dawson contributed equally to this work.
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This review considers current advances in tools to investigate the functional biology of Giardia, it's coding and non-coding genes, features and cellular and molecular biology. We consider major gaps in current knowledge of the parasite and discuss the present state-of-the-art in its in vivo and in vitro cultivation. Advances in in silico tools, including for the modelling non-coding RNAs and genomic elements, as well as detailed exploration of coding genes through inferred homology to model organisms, have provided significant, primary level insight. Improved methods to model the three-dimensional structure of proteins offer new insights into their function, and binding interactions with ligands, other proteins or precursor drugs, and offer substantial opportunities to prioritise proteins for further study and experimentation. These approaches can be supplemented by the growing and highly accessible arsenal of systems-based methods now being applied to Giardia, led by genomic, transcriptomic and proteomic methods, but rapidly incorporating advanced tools for detection of real-time transcription, evaluation of chromatin states and direct measurement of macromolecular complexes. Methods to directly interrogate and perturb gene function have made major leaps in recent years, with CRISPr-interference now available. These approaches, coupled with protein over-expression, fluorescent labelling and in vitro and in vivo imaging, are set to revolutionize the field and herald an exciting time during which the field may finally realise Giardia's long proposed potential as a model parasite and eukaryote.
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Svärd, StaffanUppsala universitet,Mikrobiologi(Swepub:uu)stsva021
(författare)
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Hagen, Kari D.Univ Calif Davis, Coll Biol Sci, Davis, CA 95616 USA.
(författare)
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Starcevich, HannahUniv Calif Davis, Coll Biol Sci, Davis, CA 95616 USA.
(författare)
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Emery-Corbin, Samantha J.Walter & Eliza Hall Inst Med Res, Populat Hlth & Immun Div, Melbourne, Vic, Australia.
(författare)
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Balan, BaluWalter & Eliza Hall Inst Med Res, Populat Hlth & Immun Div, Melbourne, Vic, Australia.
(författare)
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Nosala, ChrisUniv Calif Davis, Coll Biol Sci, Davis, CA 95616 USA.
(författare)
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Dawson, Scott C.Univ Calif Davis, Coll Biol Sci, Davis, CA 95616 USA.
(författare)
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Walter & Eliza Hall Inst Med Res, Populat Hlth & Immun Div, Melbourne, Vic, Australia.;Univ Melbourne, Fac Vet & Agr Sci, Parkville, Vic, Australia.Mikrobiologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Giardia And Giardiasis, Pt B: Elsevier, s. 97-13797801282219769780128204757
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