Sökning: onr:"swepub:oai:DiVA.org:uu-418964" > iTRAQ-Based Proteom...
Fältnamn | Indikatorer | Metadata |
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000 | 05544naa a2200505 4500 | |
001 | oai:DiVA.org:uu-418964 | |
003 | SwePub | |
008 | 200908s2020 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4189642 URI |
024 | 7 | a https://doi.org/10.3389/fmicb.2020.010842 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Alejandro Zamora-Briseno, Jesusu Inst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico.4 aut |
245 | 1 0 | a iTRAQ-Based Proteomic Profile Analysis of the Hepatopancreas of Caribbean Spiny Lobsters Infected WithPanulirus argusVirus 1 :b Metabolic and Physiological Implications |
264 | c 2020-05-29 | |
264 | 1 | b Frontiers Media SA,c 2020 |
338 | a electronic2 rdacarrier | |
520 | a The Caribbean spiny lobsterPanulirus argus(Latreille, 1084) sustains economically valuable fisheries throughout the wider Caribbean region. This species is currently affected by the pathogenic virusPanulirus argusVirus 1 (PaV1) that causes a systemic and chronic-degenerative infection in juvenile spiny lobstersP. argus. To date, there is no available information regarding the host alterations induced by this pathogen at the molecular level. In the present study, comparative proteomic analyses of the changes in the hepatopancreas between infected and non-infected juvenile lobsters were analyzed by isobaric tags for relative and absolute quantitation (iTRAQ) coupled to synchronous precursor selection (SPS)-based MS3. We identified a total of 636 proteins, being 68 down-regulated and 71 up-regulated proteins. Among the down-regulated proteins, we identified several enzymes involved in the metabolism of hormones and lipids, digestive proteases and glycosidases, while proteins associated with the histone core, protein synthesis, immune response and RNA regulation were up-regulated. Several misregulated enzymes involved in the regulation of neuromodulators were also identified. RT-qPCR assays were used to validate the expression of transcripts encoding for selected differential proteins that were in concordance to proteomic data, as well as the tendency observed in the enzymatic activities of trypsin, chymotrypsin, and glycosidase. In a similar way, we observed glycogen reduction in muscle, and an increase in plasma acylglycerides and glucose, which may be explained by proteomic data. This study provides the first insight into the molecular changes in the hepatopancreas of Caribbean spiny lobsters associated to PaV1 infection. Data provided herein would help to clarify the origin of the molecular misregulations observed at macroscopic level in this host-pathogen interaction. | |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
653 | a PaV1 | |
653 | a hepatopancreas | |
653 | a viral infection | |
653 | a proteomics | |
653 | a Panulirus argus | |
700 | 1 | a Ruiz-May, Elielu Cluster Cientif & Tecnol BioMimic, Inst Ecol, Red Estudios Mol Avanzados, Xalapa, Veracruz, Mexico.4 aut |
700 | 1 | a Miguel Elizalde-Contreras, Joseu Cluster Cientif & Tecnol BioMimic, Inst Ecol, Red Estudios Mol Avanzados, Xalapa, Veracruz, Mexico.4 aut |
700 | 1 | a Margarita Hernandez-Velazquez, Ioreniu Inst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico.4 aut |
700 | 1 | a Hernandez-Perez, Ariadneu Uppsala universitet,Jämförande fysiologi4 aut0 (Swepub:uu)arihe244 |
700 | 1 | a Guadalupe Fuentes-Garcia, Anau Inst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico.4 aut |
700 | 1 | a Herrera-Salvatierra, Nancyu Inst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico.4 aut |
700 | 1 | a Briones-Fourzan, Patriciau Univ Nacl Autonoma Mexico, Isnt Ciencias Mar & Limnol, Unidad Acad Sistemas Arrecifales, Puerto Morelos, Mexico.4 aut |
700 | 1 | a Pascual-Jimenez, Cristinau Univ Nacl Autonoma Mexico, Unidad Multidisciplinaria Docencia & Invest, Fac Ciencias, Sisal, Mexico.4 aut |
700 | 1 | a Lozano-Alvarez, Enriqueu Univ Nacl Autonoma Mexico, Isnt Ciencias Mar & Limnol, Unidad Acad Sistemas Arrecifales, Puerto Morelos, Mexico.4 aut |
700 | 1 | a Rodriguez-Canul, Rossannau Inst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico.4 aut |
710 | 2 | a Inst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico.b Cluster Cientif & Tecnol BioMimic, Inst Ecol, Red Estudios Mol Avanzados, Xalapa, Veracruz, Mexico.4 org |
773 | 0 | t Frontiers in Microbiologyd : Frontiers Media SAg 11q 11x 1664-302X |
856 | 4 | u https://doi.org/10.3389/fmicb.2020.01084y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1465004/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 | u https://www.frontiersin.org/articles/10.3389/fmicb.2020.01084/pdf |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-418964 |
856 | 4 8 | u https://doi.org/10.3389/fmicb.2020.01084 |
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